• Immunofluorescence analysis of HepG2 cells using SPG7 rabbit polyclonal antibody (STJ112287) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using SPG7 rabbit polyclonal antibody (STJ112287) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPG7 antibody (1-250) (STJ112287)

SKU:
STJ112287

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPG7 (1-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPG7
Gene ID: 6687
Uniprot ID: SPG7_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SPG7 (NP_003110.1).
Immunogen Sequence: MAVLLLLLRALRRGPGPGPR PLWGPGPAWSPGFPARPGRG RPYMASRPPGDLAEAGGRAL QSLQLRLLTPTFEGINGLLL KQHLVQNPVRLWQLLGGTFY FNTSRLKQKNKEKDKSKGKA PEEDEEERRRRERDDQMYRE RLRTLLVIAVVMSLLNALST SGGSISWNDFVHEMLAKGEV QRVQVVPESDVVEVYLHPGA VVFGRPRLALMYRMQVANID KFEEKLRAAEDELNIEAKD
Tissue Specificity Ubiquitous.
Post Translational Modifications Upon import into the mitochondrion, the N-terminal transit peptide is cleaved by the mitochondrial-processing peptidase (MPP) to generate an intermediate form which undergoes a second proteolytic cleavage mediated by proteases AFG3L1 and/or AFG3L2 removing an additional N-terminal fragment to generate the proteolytically active mature form.
Function ATP-dependent zinc metalloprotease. Plays a role in the formation and regulation of the mitochondrial permeability transition pore (mPTP) and its proteolytic activity is dispensable for this function.
Protein Name Paraplegin
Cell Matrix Adhesion Regulator
Spastic Paraplegia 7 Protein
Database Links Reactome: R-HSA-8949664
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Paraplegin antibody
Anti-Cell Matrix Adhesion Regulator antibody
Anti-Spastic Paraplegia 7 Protein antibody
Anti-SPG7 antibody
Anti-CAR antibody
Anti-CMAR antibody
Anti-PGN antibody

Information sourced from Uniprot.org

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