• Immunohistochemistry analysis of SPECC1L in paraffin-embedded Human esophageal using SPECC1L Rabbit polyclonal antibody (STJ118243) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SPECC1L Rabbit polyclonal antibody (STJ118243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-SPECC1L antibody (200-500) (STJ118243)

SKU:
STJ118243

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPECC1L (200-500) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPECC1L
Gene ID: 23384
Uniprot ID: CYTSA_HUMAN
Immunogen Region: 200-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human SPECC1L (NP_056145.4).
Immunogen Sequence: LHLRNELRDMRAQLGINEDH SEGDEKSEKETIMAHQPTDV ESTLLQLQEQNTAIREELNQ LKNENRMLKDRLNALGFSLE QRLDNSEKLFGYQSLSPEIT PGNQSDGGGTLTSSVEGSAP GSVEDLLSQDENTLMDHQHS NSMDNLDSECSEVYQPLTSS DDALDAPSSSESEGIPSIER SRKGSSGNASEVSVACLTER IHQMEENQHSTSEELQATLQ ELADLQQITQELNSENERL
Function Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration.
Protein Name Cytospin-A
Renal Carcinoma Antigen Ny-Ren-22
Sperm Antigen With Calponin Homology And Coiled-Coil Domains 1-Like
Specc1-Like Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Cell Junction
Gap Junction
Colocalizes With Acetylated Alpha-Tubulin
Gamma-Tubulin And F-Actin
Also Observed In A Ring Around Gamma-Tubulin Containing Centrioles Possibly In The Microtubule Organizing Center
Alternative Antibody Names Anti-Cytospin-A antibody
Anti-Renal Carcinoma Antigen Ny-Ren-22 antibody
Anti-Sperm Antigen With Calponin Homology And Coiled-Coil Domains 1-Like antibody
Anti-Specc1-Like Protein antibody
Anti-SPECC1L antibody
Anti-CYTSA antibody
Anti-KIAA0376 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance