| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SPC25 |
| Gene ID: | 57405 |
| Uniprot ID: | SPC25_HUMAN |
| Immunogen Region: | 120-224 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-224 of human SPC25 (NP_065726.1). |
| Immunogen Sequence: | ANKANAERLKRLQKSADLYK DRLGLEIRKIYGEKLQFIFT NIDPKNPESPFMFSLHLNEA RDYEVSDSAPHLEGLAEFQE NVRKTNNFSAFLANVRKAFT ATVYN |
| Function | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. |
| Protein Name | Kinetochore Protein Spc25 Hspc25 |
| Database Links | Reactome: R-HSA-141444 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-5663220 Reactome: R-HSA-68877 Reactome: R-HSA-9648025 |
| Cellular Localisation | Nucleus Chromosome Centromere Kinetochore Localizes To Kinetochores From Late Prophase To Anaphase Localizes Specifically To The Outer Plate Of The Kinetochore |
| Alternative Antibody Names | Anti-Kinetochore Protein Spc25 antibody Anti-Hspc25 antibody Anti-SPC25 antibody Anti-SPBC25 antibody Anti-AD024 antibody |
Information sourced from Uniprot.org