• Immunofluorescence analysis of U-2 OS cells using SPARC Rabbit polyclonal antibody (STJ116705) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L-929 cells using SPARC Rabbit polyclonal antibody (STJ116705) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SPARC Rabbit polyclonal antibody (STJ116705) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from A375 cells, using SPARC Rabbit polyclonal antibody (STJ116705) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPARC antibody (18-303) (STJ116705)

SKU:
STJ116705

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPARC (18-303) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPARC
Gene ID: 6678
Uniprot ID: SPRC_HUMAN
Immunogen Region: 18-303
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1).
Immunogen Sequence: APQQEALPDETEVVEETVAE VTEVSVGANPVQVEVGEFDD GAEETEEEVVAENPCQNHHC KHGKVCELDENNTPMCVCQD PTSCPAPIGEFEKVCSNDNK TFDSSCHFFATKCTLEGTKK GHKLHLDYIGPCKYIPPCLD SELTEFPLRMRDWLKNVLVT LYERDEDNNLLTEKQKLRVK KIHENEKRLEAGDHPVELLA RDFEKNYNMYIFPVHWQFGQ LDQHPIDGYLSHTELAPLR
Function Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites.an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Protein Name Sparc
Basement-Membrane Protein 40
Bm-40
Osteonectin
On
Secreted Protein Acidic And Rich In Cysteine
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1251985
Reactome: R-HSA-3000178
Reactome: R-HSA-3000497
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
In Or Around The Basement Membrane
Alternative Antibody Names Anti-Sparc antibody
Anti-Basement-Membrane Protein 40 antibody
Anti-Bm-40 antibody
Anti-Osteonectin antibody
Anti-On antibody
Anti-Secreted Protein Acidic And Rich In Cysteine antibody
Anti-SPARC antibody
Anti-ON antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance