• Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SPAG16 antibody (520-570 aa) (STJ195400)

SKU:
STJ195400

Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Sperm-associated antigen 16 protein (520-570 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPAG16
Gene ID: 79582
Uniprot ID: SPG16_HUMAN
Immunogen Region: 520-570 aa
Specificity: This antibody detects endogenous levels of SPG16 at Human/Mouse
Immunogen: Synthesized peptide derived from the human SPG16 at the amino acid range 520-570
Post Translational Modifications Phosphorylated by TSSK2.
Function Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia.
Protein Name Sperm-Associated Antigen 16 Protein
Pf20 Protein Homolog
Cellular Localisation Cytoplasm
Cytoskeleton
Flagellum Axoneme
Cilium Axoneme
Cell Projection
Cilium
Flagellum
Detected On The Sperm Flagellum Axoneme
Detected In The Central Apparatus Of The Axoneme
Colocalizes With Spag6 On Microtubules
Alternative Antibody Names Anti-Sperm-Associated Antigen 16 Protein antibody
Anti-Pf20 Protein Homolog antibody
Anti-SPAG16 antibody
Anti-PF20 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance