• Western blot analysis of extracts of Rat brain, using SP7 antibody (STJ11101490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Mouse bone, using SP7 antibody (STJ11101490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of U2OS cells, using SP7 antibody (STJ11101490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-SP7 antibody (333-376) (STJ11101490)

SKU:
STJ11101490

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SP7 (333-376) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SP7
Gene ID: 121340
Uniprot ID: SP7_HUMAN
Immunogen Region: 333-376
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 333-376 of human SP7 (NP_001166938.1).
Immunogen Sequence: KRFTRSDELERHVRTHTREK KFTCLLCSKRFTRSDHLSKH QRTH
Tissue Specificity Restricted to bone-derived cell.
Post Translational Modifications Ubiquitination at leads to proteasomal degradation. SP7 is a short-live protein with an endogenous half-life of approximately 12 hours. Propionylated. Depropionylation at Lys-371 by SIRT7 activates transcription factor activity and positively regulates bone formation by osteoblasts.
Function Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.
Protein Name Transcription Factor Sp7
Zinc Finger Protein Osterix
Database Links Reactome: R-HSA-8940973
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Sp7 antibody
Anti-Zinc Finger Protein Osterix antibody
Anti-SP7 antibody
Anti-OSX antibody

Information sourced from Uniprot.org

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