| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SP100 (181-480) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SP100 |
| Gene ID: |
6672 |
| Uniprot ID: |
SP100_HUMAN |
| Immunogen Region: |
181-480 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 181-480 of human SP100 (NP_001193631.1). |
| Immunogen Sequence: |
FRSLTWPPSGSPSHAGTTPP ENGLSEHPCETEQINAKRKD TTSDKDDSLGSQQTNEQCAQ KAEPTESCEQIAVQVNNGDA GREMPCPLPCDEESPEAELH NHGIQINSCSVRLVDIKKEK PFSNSKVECQAQARTHHNQA SDIIVISSEDSEGSTDVDEP LEVFISAPRSEPVINNDNPL ESNDEKEGQEATCSRPQIVP EPMDFRKLSTFRESFKKRVI GQDHDFSESSEEEAPAEAS |
| Tissue Specificity | Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines. |
| Post Translational Modifications | Sumoylated. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting. Sumoylated. Sumoylated with SUMO1. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting. Sumoylation may stabilize the interaction with CBX5. (Microbial infection) Immediate early protein IE1 of human cytomegalovirus (HHV-5) interferes with the sumoylation of SP100. |
| Function | Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. Also plays a role in infection by viruses, including human cytomegalovirus and Epstein-Barr virus, through mechanisms that may involve chromatin and/or transcriptional regulation. |
| Protein Name | Nuclear Autoantigen Sp-100Nuclear Dot-Associated Sp100 ProteinSpeckled 100 Kda |
| Database Links | Reactome: R-HSA-3108214Reactome: R-HSA-877300 |
| Cellular Localisation | NucleusPml BodyNuclear BodyCytoplasmDifferences In The Subnuclear Localization Of The Different Isoforms Seem To Exist And May Also Be Cell Cycle- And Interferon-DependentAccumulates In The Cytoplasm Upon Fas ActivationIsoform Sp100-C: NucleusForms A Reticulate Or Track-Like Nuclear Pattern With Denser Concentrations At The Nuclear Lamina And Surrounding The NucleoliA Pattern Reminiscent Of Heterochromatin-Rich Regions |
| Alternative Antibody Names | Anti-Nuclear Autoantigen Sp-100 antibodyAnti-Nuclear Dot-Associated Sp100 Protein antibodyAnti-Speckled 100 Kda antibodyAnti-SP100 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
