• Western blot analysis of various lysates using SOX30 Rabbit polyclonal antibody (STJ117993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SOX30 antibody (1-150) (STJ117993)

SKU:
STJ117993

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Sox30
Gene ID: 214105
Uniprot ID: SOX30_MOUSE
Immunogen Region: 1-150
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of mouse SOX30 (NP_775560.1).
Immunogen Sequence: MERARPEPPPPPPPPPRQPP RPTPPRPLRPAPPAQPVEAA TFRAAAAERSQSPSAQATAA MAAVASSCGEAAAAGAQAAG TRRLLQVKPEQVLLLPPGGP GVPPAPDEGAAAAAAAAAAA ASSAQARLLQLRPELLLLPP QSAADGGPCR
Tissue Specificity Expressed in the lung (at protein level). Expressed in testes (at protein level). Expressed in preleptotene spermatocytes, round spermatids, and elongated spermatids in the testis (at protein level). Expressed in pachytene spermatocytes during stages 3 to 8 of spermatogenesis (at protein level). Increased expression in diplotene spermatocytes at stage 9-11 and in metaphase spermatocytes or secondary spermatocytes at stage 12. Expressed in ovaries.
Function Acts both as a transcriptional activator and a repressor. Binds to the DNA sequence 5'-ACAAT-3' and shows a preference for guanine residues surrounding this core motif. Binds to its own promoter and activates its own transcription. Required to activate the expression of postmeiotic genes involved in spermiogenesis. Binds to the promoter region of CTNNB1 and represses its transcription which leads to inhibition of Wnt signaling. Also inhibits Wnt signaling by binding to the CTNNB1 protein, preventing interaction of CTNNB1 with TCF7L2/TCF4.
Protein Name Transcription Factor Sox-30
Cellular Localisation Nucleus
Cytoplasm
Enriched At The Chromocenter
Alternative Antibody Names Anti-Transcription Factor Sox-30 antibody
Anti-Sox30 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance