Anti-Sox2 antibody (Full-Length) [M551] (STJA0041388)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2b
ImmunogenClone M551 was generated from a recombinant protein corresponding to the full length sequence from human Sox2. This sequence is highly conserved in rat and mouse Sox2.
General Information
| Short Description | Mouse monoclonal anti-Sox2 (Full-Length) for use in ELISA, WB and ICC in Chicken, Human, Mouse, Rat and Xenopus samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC |
| Host | Mouse |
| Reactivity | Chicken/Human/Mouse/Rat/Xenopus |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M551 |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Purification | Protein G Purified |
| Dilution Range | WB 1:500ICC 1:50 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
Target Information
| Gene Symbol | SOX2 |
| Gene ID | 6657 |
| Uniprot ID | SOX2_HUMAN |
| Immunogen | Clone M551 was generated from a recombinant protein corresponding to the full length sequence from human Sox2. This sequence is highly conserved in rat and mouse Sox2. |
| Immunogen Region | Full-Length |
| Immunogen Sequence | Human |
| Specificity | This antibody antibody detects a 34 kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells, and detects a full length recombinant human Sox2 protein. |
Additional Info
| Post Translational Modifications | Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. Methylation at Lys-42 and Lys-117 is necessary for the regulation of SOX2 proteasomal degradation. Ubiquitinated by WWP2, leading to proteasomal degradation. |
| Function | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Binds to the proximal enhancer region of NANOG. Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. May function as a switch in neuronal development. |
| Protein Name | Transcription Factor Sox-2 |
| Database Links | Reactome: R-HSA-2892245Reactome: R-HSA-2892247Reactome: R-HSA-3769402Reactome: R-HSA-452723Reactome: R-HSA-6785807Reactome: R-HSA-8986944Reactome: R-HSA-9754189Reactome: R-HSA-9823739Reactome: R-HSA-9832991Reactome: R-HSA-9834899Reactome: R-HSA-9856649Reactome: R-HSA-9926550 |
| Cellular Localisation | Nucleus SpeckleCytoplasmNucleusAcetylation Contributes To Its Nuclear Localization And Deacetylation By Hdac3 Induces A Cytoplasmic DelocalizationColocalizes In The Nucleus With Znf208 Isoform Krab-O And Tyrosine Hydroxylase (Th)Colocalizes With Sox6 In SpecklesColocalizes With Caml In The NucleusNuclear Import Is Facilitated By Xpo4A Protein That Usually Acts As A Nuclear Export Signal Receptor |
| Alternative Antibody Names | Anti-Transcription Factor Sox-2 antibodyAnti-SOX2 antibody |
Information sourced from Uniprot.org