• Western blot detection of Sox2 in mES cell lysates using Sox2 antibody (1:1000 diluted).Predicted band size:35KDa, Observed band size:35KDa.Kindly provided by Dr. Qintong Li at the College of Life Sciences, Sichuan University
  • Immunocytochemistry of COS7 cells using anti-Sox2 mouse mAb diluted 1:150.
  • Flow Cytometry analysis of F9 cells stained with Sox2 (red, 1/100 dilution) , followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
  • Western blot detection of Sox2 in F9 cell lysates using Sox2 mouse mAb (1:1000 diluted).Predicted band size:35KDa.Observed band size:35KDa.

Anti-SOX2 antibody [2A10-F11-F12] (STJ99043)

SKU:
STJ99043

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/FC/ICC
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Transcription factor SOX-2 is suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 2A10-F11-F12
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
ICC 1:150
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOX2
Gene ID: 6657
Uniprot ID: SOX2_HUMAN
Specificity: This antibody detects endogenous levels of Sox2 and does not cross-react with related proteins.
Immunogen: Purified recombinant mouse Sox2 protein fragments expressed in E.coli
Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Binds to the proximal enhancer region of NANOG. Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. May function as a switch in neuronal development.
Protein Name Transcription Factor Sox-2
Database Links Reactome: R-HSA-2892245
Reactome: R-HSA-2892247
Reactome: R-HSA-3769402
Reactome: R-HSA-452723
Reactome: R-HSA-6785807
Reactome: R-HSA-8986944
Reactome: R-HSA-9754189
Cellular Localisation Nucleus Speckle
Cytoplasm
Nucleus
Acetylation Contributes To Its Nuclear Localization And Deacetylation By Hdac3 Induces A Cytoplasmic Delocalization
Colocalizes In The Nucleus With Znf208 Isoform Krab-O And Tyrosine Hydroxylase (Th)
Colocalizes With Sox6 In Speckles
Colocalizes With Caml In The Nucleus
Nuclear Import Is Facilitated By Xpo4
A Protein That Usually Acts As A Nuclear Export Signal Receptor
Alternative Antibody Names Anti-Transcription Factor Sox-2 antibody
Anti-SOX2 antibody

Information sourced from Uniprot.org

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