• Immunoprecipitation analysis of 200 Mu g extracts of C6 cells using 3 Mu g SOX2 antibody (STJ25661). Western blot was performed from the immunoprecipitate using SOX2 antibody (STJ25661) at a dilution of 1:500.
  • Immunofluorescence analysis of F9 cells using SOX2 Rabbit polyclonal antibody (STJ25661) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SOX2 in paraffin-embedded mouse embryos using SOX2 Rabbit polyclonal antibody (STJ25661) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SOX2 Rabbit polyclonal antibody (STJ25661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of lysates from MCF7 cells, using SOX2 Rabbit polyclonal antibody (STJ25661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SOX2 antibody (1-140) (STJ25661)

SKU:
STJ25661

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SOX2 (1-140) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOX2
Gene ID: 6657
Uniprot ID: SOX2_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOX2 (NP_003097.1).
Immunogen Sequence: MYNMMETELKPPGPQQTSGG GGGNSTAAAAGGNQKNSPDR VKRPMNAFMVWSRGQRRKMA QENPKMHNSEISKRLGAEWK LLSETEKRPFIDEAKRLRAL HMKEHPDYKYRPRRKTKTLM KKDKYTLPGGLLAPGGNSMA
Post Translational Modifications Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. Methylation at Lys-42 and Lys-117 is necessary for the regulation of SOX2 proteasomal degradation. Ubiquitinated by WWP2, leading to proteasomal degradation.
Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Binds to the proximal enhancer region of NANOG. Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. May function as a switch in neuronal development.
Protein Name Transcription Factor Sox-2
Database Links Reactome: R-HSA-2892245
Reactome: R-HSA-2892247
Reactome: R-HSA-3769402
Reactome: R-HSA-452723
Reactome: R-HSA-6785807
Reactome: R-HSA-8986944
Reactome: R-HSA-9754189
Cellular Localisation Nucleus Speckle
Cytoplasm
Nucleus
Acetylation Contributes To Its Nuclear Localization And Deacetylation By Hdac3 Induces A Cytoplasmic Delocalization
Colocalizes In The Nucleus With Znf208 Isoform Krab-O And Tyrosine Hydroxylase (Th)
Colocalizes With Sox6 In Speckles
Colocalizes With Caml In The Nucleus
Nuclear Import Is Facilitated By Xpo4
A Protein That Usually Acts As A Nuclear Export Signal Receptor
Alternative Antibody Names Anti-Transcription Factor Sox-2 antibody
Anti-SOX2 antibody

Information sourced from Uniprot.org

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