• Western blot analysis of extracts of HT-29 cells, using Sorbitol Dehydrogenase antibody (STJ11104612) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using Sorbitol Dehydrogenase antibody (STJ11104612) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SORD antibody (100-200) [S4612RM] (STJ11104612)

SKU:
STJ11104612

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Sorbitol Dehydrogenase (100-200) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4612RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SORD
Gene ID: 6652
Uniprot ID: DHSO_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Sorbitol Dehydrogenase (NP_003095.2).
Immunogen Sequence: RENDEFCKMGRYNLSPSIFF CATPPDDGNLCRFYKHNAAF CYKLPDNVTFEEGALIEPLS VGIHACRRGGVTLGHKVLVC GAGPIGMVTLLVAKAMGAAQ V
Tissue Specificity Expressed in liver. Expressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level).
Function Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively. Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia. May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility. May have a more general function in the metabolism of secondary alcohols since it also catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol. To a lesser extent, can also oxidize L-arabinitol, galactitol and D-mannitol and glycerol in vitro. Oxidizes neither ethanol nor other primary alcohols. Cannot use NADP(+) as the electron acceptor.
Protein Name Sorbitol Dehydrogenase
Sdh
(R -R-Butanediol Dehydrogenase
L-Iditol 2-Dehydrogenase
Polyol Dehydrogenase
Ribitol Dehydrogenase
Rdh
Xylitol Dehydrogenase
Xdh
Database Links Reactome: R-HSA-5652227
Reactome: R-HSA-5661270
Cellular Localisation Mitochondrion Membrane
Peripheral Membrane Protein
Cell Projection
Cilium
Flagellum
Associated With Mitochondria Of The Midpiece And Near The Plasma Membrane In The Principal Piece Of The Flagellum
Also Found In The Epididymosome
Secreted By The Epididymal Epithelium And That Transfers Proteins From The Epididymal Fluid To The Sperm Surface
Alternative Antibody Names Anti-Sorbitol Dehydrogenase antibody
Anti-Sdh antibody
Anti-(R -R-Butanediol Dehydrogenase antibody
Anti-L-Iditol 2-Dehydrogenase antibody
Anti-Polyol Dehydrogenase antibody
Anti-Ribitol Dehydrogenase antibody
Anti-Rdh antibody
Anti-Xylitol Dehydrogenase antibody
Anti-Xdh antibody
Anti-SORD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance