• Western blot analysis of extracts of various cell lines, using SOCS6 antibody (STJ111998) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using SOCS6 antibody (STJ111998) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human mammary cancer using SOCS6 antibody (STJ111998) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using SOCS6 antibody (STJ111998) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver cancer using SOCS6 antibody (STJ111998) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human mammary cancer using SOCS6 antibody (STJ111998) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human lung cancer using SOCS6 antibody (STJ111998) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using SOCS6 antibody (STJ111998) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SOCS6 antibody (1-210) (STJ111998)

SKU:
STJ111998

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOCS6
Gene ID: 9306
Uniprot ID: SOCS6_HUMAN
Immunogen Region: 1-210
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human SOCS6 (NP_004223.2).
Immunogen Sequence: MKKISLKTLRKSFNLNKSKE ETDFMVVQQPSLASDFGKDD SLFGSCYGKDMASCDINGED EKGGKNRSKSESLMGTLKRR LSAKQKSKGKAGTPSGSSAD EDTFSSSSAPIVFKDVRAQR PIRSTSLRSHHYSPAPWPLR PTNSEETCIKMEVRVKALVH SSSPSPALNGVRKDFHDLQS ETTCQEQANSLKSSASHNGD LHLHLDEHVP
Function SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Regulates KIT degradation by ubiquitination of the tyrosine-phosphorylated receptor.
Protein Name Suppressor Of Cytokine Signaling 6
Socs-6
Cytokine-Inducible Sh2 Protein 4
Cis-4
Suppressor Of Cytokine Signaling 4
Socs-4
Database Links Reactome: R-HSA-1433559
Reactome: R-HSA-8951664
Reactome: R-HSA-9706369
Alternative Antibody Names Anti-Suppressor Of Cytokine Signaling 6 antibody
Anti-Socs-6 antibody
Anti-Cytokine-Inducible Sh2 Protein 4 antibody
Anti-Cis-4 antibody
Anti-Suppressor Of Cytokine Signaling 4 antibody
Anti-Socs-4 antibody
Anti-SOCS6 antibody
Anti-CIS4 antibody
Anti-SOCS4 antibody

Information sourced from Uniprot.org

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