Anti-SNX3 antibody (1-162) (STJ29638)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SNX3 (1-162) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SNX3 |
| Gene ID | 8724 |
| Uniprot ID | SNX3_HUMAN |
| Immunogen Region | 1-162 |
| Immunogen Sequence | MAETVADTRRLITKPQNLND AYGPPSNFLEIDVSNPQTVG VGRGRFTTYEIRVKTNLPIF KLKESTVRRRYSDFEWLRSE LERESKVVVPPLPGKAFLRQ LPFRGDDGIFDDNFIEERKQ GLEQFINKVAGHPLAQNERC LHMFLQDEIIDKSYTPSKIR HA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human SNX3 (NP_003786.1). |
Additional Info
| Post Translational Modifications | Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP10. |
| Function | Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Can also bind phosphatidylinositol 4-phosphate (PtdIns(P4)), phosphatidylinositol 5-phosphate (PtdIns(P5)) and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2). Plays a role in protein transport between cellular compartments. Together with RAB7A facilitates endosome membrane association of the retromer cargo-selective subcomplex (CSC/VPS). May in part act as component of the SNX3-retromer complex which mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G. Not involved in EGFR degradation. Involved in the regulation of phagocytosis in dendritic cells possibly by regulating EEA1 recruitment to the nascent phagosomes. Involved in iron homeostasis through regulation of endocytic recycling of the transferrin receptor TFRC presumably by delivering the transferrin:transferrin receptor complex to recycling endosomes.the function may involve the CSC retromer subcomplex. In the case of Salmonella enterica infection plays arole in maturation of the Salmonella-containing vacuole (SCV) and promotes recruitment of LAMP1 to SCVs. |
| Protein Name | Sorting Nexin-3Protein Sdp3 |
| Database Links | Reactome: R-HSA-3238698Reactome: R-HSA-5689880 |
| Cellular Localisation | Early EndosomeCytoplasmic VesiclePhagosomeColocalizes To Clathrin-Coated Endosomal Vesicles Morphologically Distinct From Retromer-Decorated Non-Branched Endosomal Tubule Structures Colocalizes With Eea1 On Nascent Phagosomes In Dendritic Cells But Competes With Eea1 For Binding To Phagosomal MembraneIn The Case Of Salmonella Enterica Infection Localizes To Salmonella-Containing Vacuoles (Scvs) From Which Snx3-Containing Tubules Form 30-60 Minutes After Infection |
| Alternative Antibody Names | Anti-Sorting Nexin-3 antibodyAnti-Protein Sdp3 antibodyAnti-SNX3 antibody |
Information sourced from Uniprot.org