• Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using SNRPE Rabbit polyclonal antibody (STJ27441) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using SNRPE Rabbit polyclonal antibody (STJ27441) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using SNRPE Rabbit polyclonal antibody (STJ27441) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SNRPE antibody (1-92) (STJ27441)

SKU:
STJ27441

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SNRPE (1-92) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNRPE
Gene ID: 6635
Uniprot ID: RUXE_HUMAN
Immunogen Region: 1-92
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human SNRPE (NP_003085.1).
Immunogen Sequence: MAYRGQGQKVQKVMVQPINL IFRYLQNRSRIQVWLYEQVN MRIEGCIIGFDEYMNLVLDD AEEIHSKTKSRKQLGRIMLK GDNITLLQSVSN
Tissue Specificity Widely expressed. In scalp skin, it is present in the hair follicle, the epidermis, and the dermis.
Function Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. As part of the U7 snRNP it is involved in histone 3'-end processing.
Protein Name Small Nuclear Ribonucleoprotein E
Snrnp-E
Sm Protein E
Sm-E
Sme
Database Links Reactome: R-HSA-111367
Reactome: R-HSA-191859
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-73856
Reactome: R-HSA-77588
Reactome: R-HSA-9754678
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Smn-Mediated Assembly Into Core Snrnps Occurs In The Cytosol Before Smn-Mediated Transport To The Nucleus To Be Included In Spliceosomes
Alternative Antibody Names Anti-Small Nuclear Ribonucleoprotein E antibody
Anti-Snrnp-E antibody
Anti-Sm Protein E antibody
Anti-Sm-E antibody
Anti-Sme antibody
Anti-SNRPE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance