• Immunohistochemistry analysis of paraffin-embedded mouse kidney using SNRPD2 antibody (STJ29063) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using SNRPD2 antibody (STJ29063) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SNRPD2 antibody (STJ29063) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SNRPD2 antibody (1-118) (STJ29063)

SKU:
STJ29063

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SNRPD2 (1-118) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNRPD2
Gene ID: 6633
Uniprot ID: SMD2_HUMAN
Immunogen Region: 1-118
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human SNRPD2 (NP_004588.1).
Immunogen Sequence: MSLLNKPKSEMTPEELQKRE EEEFNTGPLSVLTQSVKNNT QVLINCRNNKKLLGRVKAFD RHCNMVLENVKEMWTEVPKS GKGKKKSKPVNKDRYISKMF LRGDSVIVVLRNPLIAGK
Function Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.
Protein Name Small Nuclear Ribonucleoprotein Sm D2
Sm-D2
Snrnp Core Protein D2
Database Links Reactome: R-HSA-191859
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-9754678
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Smn-Mediated Assembly Into Core Snrnps Occurs In The Cytosol Before Smn-Mediated Transport To The Nucleus To Be Included In Spliceosomes
Alternative Antibody Names Anti-Small Nuclear Ribonucleoprotein Sm D2 antibody
Anti-Sm-D2 antibody
Anti-Snrnp Core Protein D2 antibody
Anti-SNRPD2 antibody
Anti-SNRPD1 antibody

Information sourced from Uniprot.org

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