Anti-SNCA/Alpha-synuclein antibody [D10] {FITC} (STJA0028438)

SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationFITC
IsotypeIgG2ak
STJA0028438-100
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General Information

Short DescriptionMouse monoclonal Alpha-Synuclein antibody for use in ELISA, FC, IF and WB in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsELISA/FC/IF/WB
HostMouse
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO) MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS

Product Properties

ClonalityMonoclonal
Clone IDD10
IsotypeIgG2ak
ConjugationFITC
Concentration05 mg/mL
PurificationPurified
FormulationPBS pH 7.4, 50% glycerol, 0.05% proclin300
Storage InstructionStore at 4°C for 12 months Protect from light Do not freeze

Target Information

Gene SymbolSNCA
Gene ID6622
Uniprot IDSYUA_HUMAN

Additional Info

Post Translational Modifications Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress. Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers. Ubiquitinated. The predominant conjugate is the diubiquitinated form. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
Function Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.
Protein Name Alpha-Synuclein
Non-A Beta Component Of Ad Amyloid
Non-A4 Component Of Amyloid Precursor
Nacp
Database Links Reactome: R-HSA-977225
Reactome: R-HSA-9833482
Cellular Localisation Cytoplasm
Membrane
Nucleus
Synapse
Secreted
Cell Projection
Axon
Membrane-Bound In Dopaminergic Neurons
Expressed And Colocalized With Septin4 In Dopaminergic Axon Terminals
Especially At The Varicosities
Alternative Antibody Names Anti-Alpha-Synuclein antibody
Anti-Non-A Beta Component Of Ad Amyloid antibody
Anti-Non-A4 Component Of Amyloid Precursor antibody
Anti-Nacp antibody
Anti-SNCA antibody
Anti-NACP antibody
Anti-PARK1 antibody

Information sourced from Uniprot.org

Citations

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