| Short Description : | Rabbit polyclonal SMYD3 (50-150) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SMYD3 |
| Gene ID: | 64754 |
| Uniprot ID: | SMYD3_HUMAN |
| Immunogen Region: | 50-150 |
| Immunogen Sequence: | DRCLLGKEKLMRCSQCRVAK YCSAKCQKKAWPDHKRECKC LKSCKPRYPPDSVRLLGRVV FKLMDGAPSESEKLYSFYDL ESNINKLTEDKKEGLRQLVM T |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SMYD3 (NP_001161212.1). |
| Tissue Specificity | Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas. |
| Function | Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Also methylates 'Lys-5' of histone H4. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. |
| Protein Name | Histone-Lysine N-Methyltransferase Smyd3Set And Mynd Domain-Containing Protein 3Zinc Finger Mynd Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-3214841 |
| Cellular Localisation | CytoplasmNucleusMainly Cytoplasmic When Cells Are Arrested At G0/G1Accumulates In The Nucleus At S Phase And G2/M |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Smyd3 antibodyAnti-Set And Mynd Domain-Containing Protein 3 antibodyAnti-Zinc Finger Mynd Domain-Containing Protein 1 antibodyAnti-SMYD3 antibodyAnti-ZMYND1 antibodyAnti-ZNFN3A1 antibody |
Information sourced from Uniprot.org
