| Short Description : | Rabbit polyclonal SMPD4 (600-800) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:200-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SMPD4 |
| Gene ID: | 55627 |
| Uniprot ID: | NSMA3_HUMAN |
| Immunogen Region: | 600-800 |
| Immunogen Sequence: | ITQAKHTAKSISDQCAESPA GHSFLSWLGFSSMDTNGSYT ANDLDEMGQDSVRKTDEYLE KALEYLRQIFRLSEAQLRQF TLALGTTQDENGKKQLPDCI VGEDGLILTPLGRYQIINGL RRFEIEYQGDPELQPIRSYE IASLVRTLFRLSSAINHRFA GQMAALCSRDDFLGSFCRYH LTEPGLASRHLLSPVGRRQV A |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 600-800 of human SMPD4 (NP_060421.2). |
| Tissue Specificity | Widely expressed, with highest levels in heart and skeletal muscle. Isoform 1: Expressed in skeletal muscle (at protein level). Isoform 2: Expressed in skeletal muscle but a lower levels than isoform 1 (at protein level). |
| Function | Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. It has a relevant role in the homeostasis of membrane sphingolipids, thereby influencing membrane integrity, and endoplasmic reticulum organization and function. May sensitize cells to DNA damage-induced apoptosis. In skeletal muscle, mediates TNF-stimulated oxidant production. |
| Protein Name | Sphingomyelin Phosphodiesterase 4Neutral Sphingomyelinase 3Nsmase-3Nsmase3Neutral Sphingomyelinase Iii |
| Database Links | Reactome: R-HSA-9840310 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinGolgi Apparatus MembraneNucleus EnvelopeCell MembraneSarcolemma |
| Alternative Antibody Names | Anti-Sphingomyelin Phosphodiesterase 4 antibodyAnti-Neutral Sphingomyelinase 3 antibodyAnti-Nsmase-3 antibodyAnti-Nsmase3 antibodyAnti-Neutral Sphingomyelinase Iii antibodyAnti-SMPD4 antibodyAnti-KIAA1418 antibodyAnti-SKNY antibody |
Information sourced from Uniprot.org
