• Immunohistochemistry analysis of SMN1 in paraffin-embedded mouse kidney using SMN1 Rabbit polyclonal antibody (STJ118685) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SMN1 in paraffin-embedded human breast using SMN1 Rabbit polyclonal antibody (STJ118685) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SMN1 in paraffin-embedded rat lung using SMN1 Rabbit polyclonal antibody (STJ118685) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SMN1 Rabbit polyclonal antibody (STJ118685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SMN1 antibody (11-169) (STJ118685)

SKU:
STJ118685

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SMN1 (11-169) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMN1.SMN2
Gene ID: 6606/6607
Uniprot ID: SMN_HUMAN
Immunogen Region: 11-169
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 11-169 of SMN1 (NP_000335.1).
Immunogen Sequence: GVPEQEDSVLFRRGTGQSDD SDIWDDTALIKAYDKAVASF KHALKNGDICETSGKPKTTP KRKPAKKNKSQKKNTAASLQ QWKVGDKCSAIWSEDGCIYP ATIASIDFKRETCVVVYTGY GNREEQNLSDLLSPICEVAN NIEQNAQENENESQVSTDE
Tissue Specificity Expressed in a wide variety of tissues. Expressed at high levels in brain, kidney and liver, moderate levels in skeletal and cardiac muscle, and low levels in fibroblasts and lymphocytes. Also seen at high levels in spinal cord. Present in osteoclasts and mononuclear cells (at protein level).
Function The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Within the SMN complex, SMN1 acts as a structural backbone and together with GEMIN2 it gathers the Sm complex subunits. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development. Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
Protein Name Survival Motor Neuron Protein
Component Of Gems 1
Gemin-1
Database Links Reactome: R-HSA-191859
Reactome: R-HSA-9754678
Cellular Localisation Nucleus
Gem
Cajal Body
Cytoplasm
Cytoplasmic Granule
Perikaryon
Cell Projection
Neuron Projection
Axon
Myofibril
Sarcomere
Z Line
Colocalizes With Actin And At The Z-Line Of Skeletal Muscle
Under Stress Conditions Colocalizes With Rpp20/Pop7 In Punctuated Cytoplasmic Granules
Colocalized And Redistributed With Zpr1 From The Cytoplasm To Nuclear Gems (Gemini Of Coiled Bodies) And Cajal Bodies
Colocalizes With Fmr1 In Cytoplasmic Granules In The Soma And Neurite Cell Processes
Alternative Antibody Names Anti-Survival Motor Neuron Protein antibody
Anti-Component Of Gems 1 antibody
Anti-Gemin-1 antibody
Anti-SMN1 antibody
Anti-SMN antibody
Anti-SMNT.SMN2 antibody
Anti-SMNC antibody

Information sourced from Uniprot.org

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