Western blot analysis of extracts of various cell lines, using SMG5 antibody (STJ11100093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SMG5 antibody (950-1016) (STJ11100093)

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STJ11100093
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMG5
Gene ID: 23381
Uniprot ID: SMG5_HUMAN
Immunogen Region: 950-1016
Specificity: A synthetic peptide corresponding to a sequence within amino acids 950-1016 of human SMG5 (NP_056142.2).
Immunogen Sequence: DSCKQLTLAQGAGEEDPSGM VTIITGLPLDNPSVLSGPMQ AALQAAAHASVDIKNVLDFY KQWKEIG
Tissue Specificity Ubiquitous.
Function Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity.
Protein Name Nonsense-Mediated Mrna Decay Factor Smg5
Est1-Like Protein B
Lpts-Rp1
Lpts-Interacting Protein
Smg-5 Homolog
Hsmg-5
Database Links Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Predominantly Cytoplasmic
And Nuclear
Shuttles Between Nucleus And Cytoplasm
Detected In Cytoplasmic Mrna Decay Bodies
Alternative Antibody Names Anti-Nonsense-Mediated Mrna Decay Factor Smg5 antibody
Anti-Est1-Like Protein B antibody
Anti-Lpts-Rp1 antibody
Anti-Lpts-Interacting Protein antibody
Anti-Smg-5 Homolog antibody
Anti-Hsmg-5 antibody
Anti-SMG5 antibody
Anti-EST1B antibody
Anti-KIAA1089 antibody

Information sourced from Uniprot.org

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