![Western blot analysis of lysates from wild type (WT) and SMARCC1/BAF155 knockout (KO) 293T cells, using [KO Validated] SMARCC1/BAF155 Rabbit polyclonal antibody (STJ27881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101372/389062/STJ27881_2__63496.1713158665.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SMARCC1/BAF155 knockout (KO) 293T cells, using [KO Validated] SMARCC1/BAF155 Rabbit polyclonal antibody (STJ27881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Host: | Rabbit |
| Applications: | WB/IP/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SMARCC1 |
| Gene ID: | 6599 |
| Uniprot ID: | SMRC1_HUMAN |
| Immunogen Region: | 80-320 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1/BAF155 (NP_003065.3). |
| Immunogen Sequence: | LVVQLLQFQEDAFGKHVTNP AFTKLPAKCFMDFKAGGALC HILGAAYKYKNEQGWRRFDL QNPSRMDRNVEMFMNIEKTL VQNNCLTRPNIYLIPDIDLK LANKLKDIIKRHQGTFTDEK SKASHHIYPYSSSQDDEEWL RPVMRKEKQVLVHWGFYPDS YDTWVHSNDVDAEIEDPPIP EKPWKVHVKWILDTDIFNEW MNEEDYEVDENRKPVSFRQR ISTKNEEPVRSPERRDRKA |
| Tissue Specificity | Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. |
| Post Translational Modifications | Phosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein. |
| Function | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. |
| Protein Name | Swi/Snf Complex Subunit Smarcc1 Brg1-Associated Factor 155 Baf155 Swi/Snf Complex 155 Kda Subunit Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily C Member 1 |
| Database Links | Reactome: R-HSA-3214858 Reactome: R-HSA-8939243 Reactome: R-HSA-9824585 Reactome: R-HSA-9845323 |
| Cellular Localisation | Nucleus Cytoplasm |
| Alternative Antibody Names | Anti-Swi/Snf Complex Subunit Smarcc1 antibody Anti-Brg1-Associated Factor 155 antibody Anti-Baf155 antibody Anti-Swi/Snf Complex 155 Kda Subunit antibody Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily C Member 1 antibody Anti-SMARCC1 antibody Anti-BAF155 antibody |
Information sourced from Uniprot.org