Anti-SMARCA2 antibody (62-77 aa) [R02-3E2] (STJA0012593)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human SMARCA2/BRM
General Information
| Short Description | Rabbit monoclonal anti-SMARCA2 (62-77 aa) for use in WB, IHC-F, IHC-P, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R02-3E2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | SMARCA2 |
| Gene ID | 6595 |
| Uniprot ID | SMCA2_HUMAN |
| Immunogen | A synthetic peptide of human SMARCA2/BRM |
| Immunogen Region | 62-77 aa |
Additional Info
| Post Translational Modifications | During apoptosis, cleaved by cathepsin CTSG to produce a 160 kDa cleavage product which localizes to the cytosol. Ubiquitinated. |
| Function | ATPase involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. |
| Protein Name | Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 2Samrca2Brg1-Associated Factor 190bBaf190bProbable Global Transcription Activator Snf2l2Protein Brahma HomologHbrmSnf2-Alpha |
| Database Links | Reactome: R-HSA-3214858Reactome: R-HSA-8939243Reactome: R-HSA-9824585Reactome: R-HSA-9845323 |
| Cellular Localisation | NucleusLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 2 antibodyAnti-Samrca2 antibodyAnti-Brg1-Associated Factor 190b antibodyAnti-Baf190b antibodyAnti-Probable Global Transcription Activator Snf2l2 antibodyAnti-Protein Brahma Homolog antibodyAnti-Hbrm antibodyAnti-Snf2-Alpha antibodyAnti-SMARCA2 antibodyAnti-BAF190B antibodyAnti-BRM antibodyAnti-SNF2A antibodyAnti-SNF2L2 antibody |
Information sourced from Uniprot.org