| Short Description : | Goat polyclonal antibody anti-SMAD9 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications: | Pep-ELISA/WB/IHC |
| Host: | Goat |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 50kDa band observed in Human Skin lysates (calculated MW of 48.6kDa according to NP_005896.1). Recommended concentration: 0.3-1.0 µg/ml. Primary incubation was 1 hour.IHC: |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | SMAD9 |
| Gene ID: | 4093 |
| Uniprot ID: | SMAD9_HUMAN |
| Accession Number: | NP_005896.1 |
| Immunogen Region: | Internal |
| Immunogen Sequence: | HSEPLMPHNATYPD |
| Specificity: | This antibody is not expected to cross-react with SMAD1 and SMAD5. |
| Post Translational Modifications | Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD). |
| Protein Name | Mothers Against Decapentaplegic Homolog 9Mad Homolog 9Mothers Against Dpp Homolog 9Madh6Smad Family Member 9Smad 9Smad9 |
| Database Links | Reactome: R-HSA-201451 |
| Cellular Localisation | CytoplasmNucleusIn The Cytoplasm In The Absence Of LigandMigration To The Nucleus When Complexed With Smad4 |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 9 antibodyAnti-Mad Homolog 9 antibodyAnti-Mothers Against Dpp Homolog 9 antibodyAnti-Madh6 antibodyAnti-Smad Family Member 9 antibodyAnti-Smad 9 antibodyAnti-Smad9 antibodyAnti-SMAD9 antibodyAnti-MADH6 antibodyAnti-MADH9 antibodyAnti-SMAD8 antibody |
Information sourced from Uniprot.org
