![Western blot analysis of lysates from wild type (WT) and Smad5 knockout (KO) 293T (KO) cells, using [KO Validated] Smad5 Rabbit monoclonal antibody (STJ11104940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177584/369430/STJ11104940_1__68210.1713134930.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Smad5 knockout (KO) 293T (KO) cells, using [KO Validated] Smad5 Rabbit monoclonal antibody (STJ11104940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western blot analysis of lysates from wild type (WT) and Smad5 knockout (KO) 293T (KO) cells, using [KO Validated] Smad5 Rabbit monoclonal antibody (STJ11104940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/177584/369430/STJ11104940_1__68210.1713134930.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Smad5 knockout (KO) 293T (KO) cells, using [KO Validated] Smad5 Rabbit monoclonal antibody (STJ11104940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
Anti-SMAD5 antibody (186-260) [S4940RM] (STJ11104940)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Mothers Against Decapentaplegic Homolog 5 (186-260) antibody for use in WB and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4940RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SMAD5 |
| Gene ID | 4090 |
| Uniprot ID | SMAD5_HUMAN |
| Immunogen Region | 186-260 |
| Immunogen Sequence | PLSPNSPYPPSPASSTYPNS PASSGPGSPFQLPADTPPPA YMPPDDQMGQDNSQPMDTSN NMIPQIMPSISSRDV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 186-260 of human Smad5 (NP_005894.3). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. |
| Function | Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis. |
| Protein Name | Mothers Against Decapentaplegic Homolog 5Mad Homolog 5Mothers Against Dpp Homolog 5Jv5-1Smad Family Member 5Smad 5Smad5Hsmad5 |
| Database Links | Reactome: R-HSA-201451 |
| Cellular Localisation | CytoplasmNucleusMitochondrionCytoplasmic In The Absence Of LigandMigrates To The Nucleus When Complexed With Smad4 |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 5 antibodyAnti-Mad Homolog 5 antibodyAnti-Mothers Against Dpp Homolog 5 antibodyAnti-Jv5-1 antibodyAnti-Smad Family Member 5 antibodyAnti-Smad 5 antibodyAnti-Smad5 antibodyAnti-Hsmad5 antibodyAnti-SMAD5 antibodyAnti-MADH5 antibody |
Information sourced from Uniprot.org