| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Smad4 (160-450) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SMAD4 |
| Gene ID: |
4089 |
| Uniprot ID: |
SMAD4_HUMAN |
| Immunogen Region: |
160-450 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 160-450 of human Smad4 (NP_005350.1). |
| Immunogen Sequence: |
DEYVHDFEGQPSLSTEGHSI QTIQHPPSNRASTETYSTPA LLAPSESNATSTANFPNIPV ASTSQPASILGGSHSEGLLQ IASGPQPGQQQNGFTGQPAT YHHNSTTTWTGSRTAPYTPN LPHHQNGHLQHHPPMPPHPG HYWPVHNELAFQPPISNHPA PEYWCSIAYFEMDVQVGETF KVPSSCPIVTVDGYVDPSGG DRFCLGQLSNVHRTEAIERA RLHIGKGVQLECKGEGDVW |
| Post Translational Modifications | Phosphorylated by PDPK1. Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination by USP9X restores its competence to mediate TGF-beta signaling. |
| Function | In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site.required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Protein Name | Mothers Against Decapentaplegic Homolog 4Mad Homolog 4Mothers Against Dpp Homolog 4Deletion Target In Pancreatic Carcinoma 4Smad Family Member 4Smad 4Smad4Hsmad4 |
| Database Links | Reactome: R-HSA-1181150Reactome: R-HSA-1502540Reactome: R-HSA-201451Reactome: R-HSA-2173789Reactome: R-HSA-2173795Reactome: R-HSA-2173796Reactome: R-HSA-3311021Reactome: R-HSA-3315487Reactome: R-HSA-452723Reactome: R-HSA-5689880Reactome: R-HSA-8941326Reactome: R-HSA-8941855Reactome: R-HSA-8952158Reactome: R-HSA-9615017Reactome: R-HSA-9617828Reactome: R-HSA-9733709Reactome: R-HSA-9735871Reactome: R-HSA-9754189Reactome: R-HSA-9823730 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic In The Absence Of LigandMigrates To The Nucleus When Complexed With R-SmadPdpk1 Prevents Its Nuclear Translocation In Response To Tgf-Beta |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 4 antibodyAnti-Mad Homolog 4 antibodyAnti-Mothers Against Dpp Homolog 4 antibodyAnti-Deletion Target In Pancreatic Carcinoma 4 antibodyAnti-Smad Family Member 4 antibodyAnti-Smad 4 antibodyAnti-Smad4 antibodyAnti-Hsmad4 antibodyAnti-SMAD4 antibodyAnti-DPC4 antibodyAnti-MADH4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Smad4 Rabbit polyclonal antibody knockdown (KD) 293T cells, using [KD Validated] Smad4 Rabbit polyclonal antibody (STJ11104369) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/177013/368191/STJ11104369_1__05410.1713133699.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Smad4 Rabbit polyclonal antibody knockdown (KD) 293T cells, using [KD Validated] Smad4 Rabbit polyclonal antibody (STJ11104369) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
