• Immunohistochemistry analysis of SLIRP in paraffin-embedded Human colon carcinoma using SLIRP Rabbit polyclonal antibody (STJ117719) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SLIRP Rabbit polyclonal antibody (STJ117719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SLIRP antibody (1-100) (STJ117719)

SKU:
STJ117719

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLIRP (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLIRP
Gene ID: 81892
Uniprot ID: SLIRP_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SLIRP (NP_112487.1).
Immunogen Sequence: MAASAARGAAALRRSINQPV AFVRRIPWTAASSQLKEHFA QFGHVRRCILPFDKETGFHR GLGWVQFSSEEGLRNALQQE NHIIDGVKVQVHTRRPKLPQ
Tissue Specificity Ubiquitously expressed, with highest level in heart, liver, skeletal muscle and testis.
Function RNA-binding protein that acts as a nuclear receptor corepressor. Probably acts by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. Binds the STR7 loop of SRA RNA. Also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation.
Protein Name Sra Stem-Loop-Interacting Rna-Binding Protein - Mitochondrial
Cellular Localisation Mitochondrion
Nucleus
Predominantly Mitochondrial
Some Fraction Is Nuclear
In The Nucleus
It Is Recruited To Nuclear Receptor Target Promoters
Alternative Antibody Names Anti-Sra Stem-Loop-Interacting Rna-Binding Protein - Mitochondrial antibody
Anti-SLIRP antibody
Anti-C14orf156 antibody
Anti-DC23 antibody
Anti-DC50 antibody
Anti-PD04872 antibody

Information sourced from Uniprot.org

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