| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SLC5A7 (500-580 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB (confirmed by recombinant protein). A dilution of 1: 200 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
SLC5A7 |
| Gene ID: |
60482 |
| Uniprot ID: |
SC5A7_HUMAN |
| Immunogen Region: |
500-580 aa |
| Specificity: |
Specific for SLC5A7. |
| Immunogen: |
A synthetic peptide from aa region 500-580 of human SLC5A7 conjugated to blue carrier protein was used as the antigen. |
| Post Translational Modifications | Phosphorylated. |
| Function | High-affinity Na(+)-coupled choline transmembrane symporter. Functions as an electrogenic, voltage-dependent transporter with variable charge/choline stoichiometry. Choline uptake and choline-induced current is also Cl(-)-dependent where Cl(-) is likely a regulatory ion rather than cotransported ion. Plays a critical role in acetylcholine (ACh) synthesis by taking up the substrate choline from the synaptic cleft into the presynaptic nerve terminals after neurotransmitter release. SLC5A7/CHT1-mediated choline high-affinity transport in cholinergic neurons is the rate-limiting step for production of ACh, thereby facilitating communication by subsequent action potentials. Localized predominantly in presynaptic terminal intracellular organelles, and translocated to the plasma membrane in active form in response to neuronal activity. |
| Protein Name | High Affinity Choline Transporter 1Hcht1Hemicholinium-3-Sensitive Choline TransporterChtSolute Carrier Family 5 Member 7 |
| Database Links | Reactome: R-HSA-264642Reactome: R-HSA-425366Reactome: R-HSA-5619114Reactome: R-HSA-5658471 |
| Cellular Localisation | Presynaptic Cell MembraneMulti-Pass Membrane ProteinCell ProjectionAxonEarly Endosome MembraneCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneLocalized At The Neuromuscular JunctionLocalization At The Plasma Membrane Is Transient Due To The Rapid Endocytosis Of Slc5a7/Cht1 Via The Clathrin-Mediated PathwayWhere It Localizes To Early EndosomesAlso Localized To Synaptic Vesicles Where It Is Likely Mobilized To The Cell Surface By Exocytosis |
| Alternative Antibody Names | Anti-High Affinity Choline Transporter 1 antibodyAnti-Hcht1 antibodyAnti-Hemicholinium-3-Sensitive Choline Transporter antibodyAnti-Cht antibodyAnti-Solute Carrier Family 5 Member 7 antibodyAnti-SLC5A7 antibodyAnti-CHT1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
