Anti-SLC5A7 antibody (149-199) (STJA0006310)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human SC5A7 AA range: 149-199
General Information
| Short Description | Rabbit polyclonal anti-High affinity choline transporter 1 (149-199) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC5A7 |
| Gene ID | 60482 |
| Uniprot ID | SC5A7_HUMAN |
| Immunogen | Synthesized peptide derived from human SC5A7 AA range: 149-199 |
| Immunogen Region | 149-199 |
| Specificity | This antibody detects endogenous levels of human SC5A7 |
Additional Info
| Post Translational Modifications | Phosphorylated. |
| Function | High-affinity Na(+)-coupled choline transmembrane symporter. Functions as an electrogenic, voltage-dependent transporter with variable charge/choline stoichiometry. Choline uptake and choline-induced current is also Cl(-)-dependent where Cl(-) is likely a regulatory ion rather than cotransported ion. Plays a critical role in acetylcholine (ACh) synthesis by taking up the substrate choline from the synaptic cleft into the presynaptic nerve terminals after neurotransmitter release. SLC5A7/CHT1-mediated choline high-affinity transport in cholinergic neurons is the rate-limiting step for production of ACh, thereby facilitating communication by subsequent action potentials. Localized predominantly in presynaptic terminal intracellular organelles, and translocated to the plasma membrane in active form in response to neuronal activity. |
| Protein Name | High Affinity Choline Transporter 1Hcht1Hemicholinium-3-Sensitive Choline TransporterChtSolute Carrier Family 5 Member 7 |
| Database Links | Reactome: R-HSA-264642Reactome: R-HSA-425366Reactome: R-HSA-5619114Reactome: R-HSA-5658471 |
| Cellular Localisation | Presynaptic Cell MembraneMulti-Pass Membrane ProteinCell ProjectionAxonEarly Endosome MembraneCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneLocalized At The Neuromuscular JunctionLocalization At The Plasma Membrane Is Transient Due To The Rapid Endocytosis Of Slc5a7/Cht1 Via The Clathrin-Mediated PathwayWhere It Localizes To Early EndosomesAlso Localized To Synaptic Vesicles Where It Is Likely Mobilized To The Cell Surface By Exocytosis |
| Alternative Antibody Names | Anti-High Affinity Choline Transporter 1 antibodyAnti-Hcht1 antibodyAnti-Hemicholinium-3-Sensitive Choline Transporter antibodyAnti-Cht antibodyAnti-Solute Carrier Family 5 Member 7 antibodyAnti-SLC5A7 antibodyAnti-CHT1 antibody |
Information sourced from Uniprot.org