• Western blot analysis of various lysates, using SLC5A2 rabbit polyclonal antibody (STJ11103251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using SLC5A2 rabbit polyclonal antibody (STJ11103251) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using SLC5A2 rabbit polyclonal antibody (STJ11103251) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Rat kidney using SLC5A2 antibody (STJ11103251) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Human kidney using SLC5A2 antibody (STJ11103251) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat kidney cells using SLC5A2 rabbit polyclonal antibody (STJ11103251) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SLC5A2 antibody (564-624) (STJ11103251)

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STJ11103251

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC5A2 (564-624) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC5A2
Gene ID: 6524
Uniprot ID: SC5A2_HUMAN
Immunogen Region: 564-624
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 564-624 of human SLC5A2 (NP_003032.1).
Immunogen Sequence: RHSKEEREDLDADEQQGSSL PVQNGCPESAMEMNEPQAPA PSLFRQCLLWFCGMSRGGVG S
Function Electrogenic Na(+)-coupled sugar simporter that actively transports D-glucose at the plasma membrane, with a Na(+) to sugar coupling ratio of 1:1. Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump. Has a primary role in D-glucose reabsorption from glomerular filtrate across the brush border of the early proximal tubules of the kidney.
Protein Name Sodium/Glucose Cotransporter 2
Na(+/Glucose Cotransporter 2
Low Affinity Sodium-Glucose Cotransporter
Solute Carrier Family 5 Member 2
Database Links Reactome: R-HSA-189200
Reactome: R-HSA-5658208
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Sodium/Glucose Cotransporter 2 antibody
Anti-Na(+/Glucose Cotransporter 2 antibody
Anti-Low Affinity Sodium-Glucose Cotransporter antibody
Anti-Solute Carrier Family 5 Member 2 antibody
Anti-SLC5A2 antibody
Anti-SGLT2 antibody

Information sourced from Uniprot.org

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