• Immunohistochemistry analysis of paraffin-embedded human liver using SLC4A5 antibody (STJ112467) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using SLC4A5 antibody (STJ112467) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using SLC4A5 antibody (STJ112467) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SLC4A5 antibody (STJ112467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-SLC4A5 antibody (1042-1121) (STJ112467)

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STJ112467

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC4A5 (1042-1121) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC4A5
Gene ID: 57835
Uniprot ID: S4A5_HUMAN
Immunogen Region: 1042-1121
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1042-1121 of human SLC4A5 (NP_597812.1).
Immunogen Sequence: DFIFSQHDLAWIDNILPEKE KKETDKKRKRKKGAHEDCDE EPQFPPPSVIKIPMESVQSD PQNGIHCIARKRSSSWSYSL
Tissue Specificity Highest expression observed in liver, spleen and testis.moderate expression in the choroid plexus, hippocampus, cerebrum and cerebellum of brain, and in kidney cortex and kidney medulla. Also observed in heart, pancreas, muscle, lung, placenta, stomach and small intestine. Weakest expression seen in peripheral blood lymphocytes, colon, duodenum, jejunum, ileum and skeletal muscle. Isoform 3: Expressed in the brain, heart, kidney, testis, pancreas, muscle and peripheral blood leukocytes.
Function Mediates sodium- and bicarbonate-dependent electrogenic sodium bicarbonate cotransport, with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3.
Protein Name Electrogenic Sodium Bicarbonate Cotransporter 4
Nbce2
Solute Carrier Family 4 Member 5
Database Links Reactome: R-HSA-425381
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Expressed In The Apical Plasma Membrane Domain Of A Subset Of Collecting Ducts In The Renal Medulla
Alternative Antibody Names Anti-Electrogenic Sodium Bicarbonate Cotransporter 4 antibody
Anti-Nbce2 antibody
Anti-Solute Carrier Family 4 Member 5 antibody
Anti-SLC4A5 antibody
Anti-NBC4 antibody

Information sourced from Uniprot.org

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