• Immunofluorescence analysis of C6 cells using SLC4A4/ NBC Rabbit polyclonal antibody (STJ27285) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SLC4A4/ NBC Rabbit polyclonal antibody (STJ27285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of lysates from LNCaP cells, using SLC4A4/ NBC Rabbit polyclonal antibody (STJ27285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SLC4A4 antibody (978-1078) (STJ27285)

SKU:
STJ27285

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC4A4/NBC (978-1078) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC4A4
Gene ID: 8671
Uniprot ID: S4A4_HUMAN
Immunogen Region: 978-1078
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 978-1078 of human SLC4A4/NBC (NP_001091954.1).
Immunogen Sequence: VMILALVAVRKGMDYLFSQH DLSFLDDVIPEKDKKKKEDE KKKKKKKGSLDSDNDDSDCP YSEKVPSIKIPMDIMEQQPF LSDSKPSDRERSPTFLERHT S
Tissue Specificity Isoform 1: Expressed in the corneal endothelium cells (at protein level). Expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Isoform 2: Specifically expressed in kidney at the level of proximal tubules.
Post Translational Modifications Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylated in presence of STK39 and dephosphorylated in presence of PP1 phosphatase.phosphorylation seems to inhibit SLC4A4 activity. N-glycosylated. May not be necessary for the transporter basic functions.
Function Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
Protein Name Electrogenic Sodium Bicarbonate Cotransporter 1
Sodium Bicarbonate Cotransporter
Na(+/Hco3(- Cotransporter
Solute Carrier Family 4 Member 4
Knbc1
Database Links Reactome: R-HSA-425381
Reactome: R-HSA-5619054
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Electrogenic Sodium Bicarbonate Cotransporter 1 antibody
Anti-Sodium Bicarbonate Cotransporter antibody
Anti-Na(+/Hco3(- Cotransporter antibody
Anti-Solute Carrier Family 4 Member 4 antibody
Anti-Knbc1 antibody
Anti-SLC4A4 antibody
Anti-NBC antibody
Anti-NBC1 antibody
Anti-NBCE1 antibody

Information sourced from Uniprot.org

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