| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Proton-Coupled Folate Transporter (1-130) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLC46A1 |
| Gene ID: |
113235 |
| Uniprot ID: |
PCFT_HUMAN |
| Immunogen Region: |
1-130 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SLC46A1 (NP_001229295.1). |
| Immunogen Sequence: |
MEGSASPPEKPRARPAAAVL CRGPVEPLVFLANFALVLQG PLTTQYLWHRFSADLGYNGT RQRGGCSNRSADPTMQEVET LTSHWTLYMNVGGFLVGLFS STLLGAWSDSVGRRPLLVLA SLGLLLQALV |
| Tissue Specificity | Expressed at highest level in the upper half of the small intestine (duodenum and jejunum), expression decreases downwardly in the subsequent quarter and is undetectable in the last quarter (the lowest ileum). Also expressed in kidney, liver, placenta, spleen, retina and retinal pigment epithelium. Lower levels found in testis. Very low levels in brain, lung, stomach, heart and muscle. |
| Function | Proton-coupled folate symporter that mediates folate absorption using an H(+) gradient as a driving force. Involved in the intestinal absorption of folates at the brush-border membrane of the proximal jejunum, and the transport from blood to cerebrospinal fluid across the choroid plexus. Functions at acidic pH via alternate outward- and inward-open conformation states. Protonation of residues in the outward open state primes the protein for transport. Binding of folate promotes breaking of salt bridge network and subsequent closure of the extracellular gate, leading to the inward-open state and release of protons and folate. Also able to transport antifolate drugs, such as methotrexate and pemetrexed, which are established treatments for cancer and autoimmune diseases. Involved in FOLR1-mediated endocytosis by serving as a route of export of folates from acidified endosomes. Also acts as a lower-affinity, pH-independent heme carrier protein and constitutes the main importer of heme in the intestine. Imports heme in the retina and retinal pigment epithelium, in neurons of the hippocampus, in hepatocytes and in the renal epithelial cells. Hence, participates in the trafficking of heme and increases intracellular iron content. Isoform 2: Inactive isoform which is not able to mediate proton-coupled folate transport. |
| Protein Name | Proton-Coupled Folate TransporterHspcftHpcftHeme Carrier Protein 1Pcft/Hcp1Solute Carrier Family 46 Member 1 |
| Database Links | Reactome: R-HSA-196757Reactome: R-HSA-917937Reactome: R-HSA-9707616 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinApical Cell MembraneBasolateral Cell MembraneEndosome MembraneCytoplasmLocalizes To The Apical Membrane Of Intestinal Cells In Iron-Deficient CellsWhile It Resides In The Cytoplasm In Iron-Replete CellsLocalizes To The Basolateral Membrane Of Choroid Plexus |
| Alternative Antibody Names | Anti-Proton-Coupled Folate Transporter antibodyAnti-Hspcft antibodyAnti-Hpcft antibodyAnti-Heme Carrier Protein 1 antibodyAnti-Pcft/Hcp1 antibodyAnti-Solute Carrier Family 46 Member 1 antibodyAnti-SLC46A1 antibodyAnti-G21 antibodyAnti-HCP1 antibodyAnti-PCFT antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
