• Western blot analysis of various lysates, using SLC46A1 Rabbit polyclonal antibody (STJ11105729) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-SLC46A1 antibody (1-130) (STJ11105729)

SKU:
STJ11105729

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Proton-Coupled Folate Transporter (1-130) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC46A1
Gene ID: 113235
Uniprot ID: PCFT_HUMAN
Immunogen Region: 1-130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SLC46A1 (NP_001229295.1).
Immunogen Sequence: MEGSASPPEKPRARPAAAVL CRGPVEPLVFLANFALVLQG PLTTQYLWHRFSADLGYNGT RQRGGCSNRSADPTMQEVET LTSHWTLYMNVGGFLVGLFS STLLGAWSDSVGRRPLLVLA SLGLLLQALV
Tissue Specificity Expressed at highest level in the upper half of the small intestine (duodenum and jejunum), expression decreases downwardly in the subsequent quarter and is undetectable in the last quarter (the lowest ileum). Also expressed in kidney, liver, placenta, spleen, retina and retinal pigment epithelium. Lower levels found in testis. Very low levels in brain, lung, stomach, heart and muscle.
Function Proton-coupled folate symporter that mediates folate absorption using an H(+) gradient as a driving force. Involved in the intestinal absorption of folates at the brush-border membrane of the proximal jejunum, and the transport from blood to cerebrospinal fluid across the choroid plexus. Functions at acidic pH via alternate outward- and inward-open conformation states. Protonation of residues in the outward open state primes the protein for transport. Binding of folate promotes breaking of salt bridge network and subsequent closure of the extracellular gate, leading to the inward-open state and release of protons and folate. Also able to transport antifolate drugs, such as methotrexate and pemetrexed, which are established treatments for cancer and autoimmune diseases. Involved in FOLR1-mediated endocytosis by serving as a route of export of folates from acidified endosomes. Also acts as a lower-affinity, pH-independent heme carrier protein and constitutes the main importer of heme in the intestine. Imports heme in the retina and retinal pigment epithelium, in neurons of the hippocampus, in hepatocytes and in the renal epithelial cells. Hence, participates in the trafficking of heme and increases intracellular iron content. Isoform 2: Inactive isoform which is not able to mediate proton-coupled folate transport.
Protein Name Proton-Coupled Folate Transporter
Hspcft
Hpcft
Heme Carrier Protein 1
Pcft/Hcp1
Solute Carrier Family 46 Member 1
Database Links Reactome: R-HSA-196757
Reactome: R-HSA-917937
Reactome: R-HSA-9707616
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Basolateral Cell Membrane
Endosome Membrane
Cytoplasm
Localizes To The Apical Membrane Of Intestinal Cells In Iron-Deficient Cells
While It Resides In The Cytoplasm In Iron-Replete Cells
Localizes To The Basolateral Membrane Of Choroid Plexus
Alternative Antibody Names Anti-Proton-Coupled Folate Transporter antibody
Anti-Hspcft antibody
Anti-Hpcft antibody
Anti-Heme Carrier Protein 1 antibody
Anti-Pcft/Hcp1 antibody
Anti-Solute Carrier Family 46 Member 1 antibody
Anti-SLC46A1 antibody
Anti-G21 antibody
Anti-HCP1 antibody
Anti-PCFT antibody

Information sourced from Uniprot.org

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