• STJ73127 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73127 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-SLC40A1 antibody (245-259) (STJ73127)

SKU:
STJ73127-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SLC40A1 (245-259) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Preliminary testing showed a band at approx 60kDa in Human Breast and Colonr lysate after 1-2µg/ml antibody staining (calculated MW of 62.5kDa according to NP_055400.1). Primary incub
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SLC40A1
Gene ID: 30061
Uniprot ID: S40A1_HUMAN
Immunogen Region: 245-259
Accession Number: NP_055400.1
Immunogen Sequence: ELKQLNLHKDTEPKP
Post Translational Modifications Polyubiquitinated by RNF217.leading to proteasomal degradation. Under conditions of high systemic iron levels, both the hormone peptide hepcidin/HAMP and holo(iron bound)-transferrin/TF induce the ubiquitination, internalization and proteasomal degradation of SLC40A1 to control iron release from cells.
Function Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis. Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues. Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes. When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma.
Protein Name Ferroportin
Ferroportin-1
Iron-Regulated Transporter 1
Solute Carrier Family 40 Member 1
Database Links Reactome: R-HSA-425410
Reactome: R-HSA-5619049
Reactome: R-HSA-5619060
Reactome: R-HSA-5655799
Reactome: R-HSA-917937
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Localized To The Basolateral Membrane Of Hepatocytoma Wif-B Cells
Alternative Antibody Names Anti-Ferroportin antibody
Anti-Ferroportin-1 antibody
Anti-Iron-Regulated Transporter 1 antibody
Anti-Solute Carrier Family 40 Member 1 antibody
Anti-SLC40A1 antibody
Anti-FPN antibody
Anti-FPN1 antibody
Anti-IREG1 antibody
Anti-SLC11A3 antibody
Anti-MSTP079 antibody

Information sourced from Uniprot.org

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