Anti-SLC40A1 antibody (200-270) (STJ117085)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SLC40A1 (200-270) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC40A1 |
| Gene ID | 30061 |
| Uniprot ID | S40A1_HUMAN |
| Immunogen Region | 200-270 |
| Immunogen Sequence | SPVIGCGFISGWNLVSMCVE YVLLWKVYQKTPALAVKAGL KEEETELKQLNLHKDTEPKP LEGTHLMGVKD |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 200-270 of human SLC40A1 (NP_055400.1). |
Additional Info
| Tissue Specificity | Detected in erythrocytes (at protein level). Expressed in placenta, intestine, muscle and spleen. Highly expressed in mature red blood. |
| Post Translational Modifications | Polyubiquitinated by RNF217.leading to proteasomal degradation. Under conditions of high systemic iron levels, both the hormone peptide hepcidin/HAMP and holo(iron bound)-transferrin/TF induce the ubiquitination, internalization and proteasomal degradation of SLC40A1 to control iron release from cells. |
| Function | Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis. Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues. Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes. When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma. |
| Protein Name | FerroportinFerroportin-1Iron-Regulated Transporter 1Solute Carrier Family 40 Member 1 |
| Database Links | Reactome: R-HSA-425410Reactome: R-HSA-5619049Reactome: R-HSA-5619060Reactome: R-HSA-5655799Reactome: R-HSA-917937 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneLocalized To The Basolateral Membrane Of Hepatocytoma Wif-B Cells |
| Alternative Antibody Names | Anti-Ferroportin antibodyAnti-Ferroportin-1 antibodyAnti-Iron-Regulated Transporter 1 antibodyAnti-Solute Carrier Family 40 Member 1 antibodyAnti-SLC40A1 antibodyAnti-FPN antibodyAnti-FPN1 antibodyAnti-IREG1 antibodyAnti-SLC11A3 antibodyAnti-MSTP079 antibody |
Information sourced from Uniprot.org