| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ZIP8 (180-300) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLC39A8 |
| Gene ID: |
64116 |
| Uniprot ID: |
S39A8_HUMAN |
| Immunogen Region: |
180-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 180-300 of human ZIP8 (NP_071437.3). |
| Immunogen Sequence: |
QLIPEAFGFDPKVDSYVEKA VAVFGGFYLLFFFERMLKML LKTYGQNGHTHFGNDNFGPQ EKTHQPKALPAINGVTCYAN PAVTEANGHIHFDNVSVVSL QDGKKEPSSCTCLKGPKLSE I |
| Tissue Specificity | Ubiquitously expressed. Expressed in thymus, placenta, lung, liver, pancreas, salivary gland and, to a lower extent, in spleen, testis, ovary, small intestine, colon, leukocyte, heart. Highest expression is observed in pancreas. Expressed by macrophages (at protein level). Expressed by microvascular capillary endothelial cells that constitute the blood-brain barrier (at protein level). |
| Post Translational Modifications | N-glycosylated. N-glycosylation is not required for proper iron and zinc transport. |
| Function | Electroneutral divalent metal cation:bicarbonate symporter of the plasma membrane mediating the cellular uptake of zinc and manganese, two divalent metal cations important for development, tissue homeostasis and immunity. Transports an electroneutral complex composed of a divalent metal cation and two bicarbonate anions or alternatively a bicarbonate and a selenite anion. Thereby, it also contributes to the cellular uptake of selenium, an essential trace metal and micronutrient. Also imports cadmium a non-essential metal which is cytotoxic and carcinogenic. May also transport iron and cobalt through membranes. Through zinc import, indirectly regulates the metal-dependent transcription factor MTF1 and the expression of some metalloproteases involved in cartilage catabolism and also probably heart development. Also indirectly regulates the expression of proteins involved in cell morphology and cytoskeleton organization. Indirectly controls innate immune function and inflammatory response by regulating zinc cellular uptake which in turn modulates the expression of genes specific of these processes. Protects, for instance, cells from injury and death at the onset of inflammation. By regulating zinc influx into monocytes also directly modulates their adhesion to endothelial cells and arteries. Reclaims manganese from the bile at the apical membrane of hepatocytes, thereby regulating the activity of the manganese-dependent enzymes through the systemic levels of the nutrient. Also participates in manganese reabsorption in the proximal tubule of the kidney. By mediating the extracellular uptake of manganese by cells of the blood-brain barrier, may also play a role in the transport of the micronutrient to the brain. With manganese cellular uptake also participates in mitochondrial proper function. Finally, also probably functions intracellularly, translocating zinc from lysosome to cytosol to indirectly enhance the expression of specific genes during TCR-mediated T cell activation. |
| Protein Name | Metal Cation Symporter Zip8Bcg-Induced Integral Membrane Protein In Monocyte Clone 103 ProteinLiv-1 Subfamily Of Zip Zinc Transporter 6Lzt-Hs6Solute Carrier Family 39 Member 8Zrt- And Irt-Like Protein 8Zip-8 |
| Database Links | Reactome: R-HSA-442380 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinLysosome MembraneApical Cell MembraneBasolateral Cell MembraneLocalizes To The Lysosome Of Activated T-CellsA Large Fraction Of The Protein Is Found Intracellularly In Microvascular Capillary Endothelial Cells That Constitute The Blood-Brain BarrierLocalized And Functional At Both Apical And Basolateral Membranes Of Microvascular Capillary Endothelial Cells That Constitute The Blood-Brain Barrier |
| Alternative Antibody Names | Anti-Metal Cation Symporter Zip8 antibodyAnti-Bcg-Induced Integral Membrane Protein In Monocyte Clone 103 Protein antibodyAnti-Liv-1 Subfamily Of Zip Zinc Transporter 6 antibodyAnti-Lzt-Hs6 antibodyAnti-Solute Carrier Family 39 Member 8 antibodyAnti-Zrt- And Irt-Like Protein 8 antibodyAnti-Zip-8 antibodyAnti-SLC39A8 antibodyAnti-BIGM103 antibodyAnti-ZIP8 antibodyAnti-PP3105 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
