• Immunofluorescence analysis of L929 cells using ZIP8 Rabbit polyclonal antibody (STJ112431) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ZIP8 antibody (STJ112431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SLC39A8 antibody (180-300) (STJ112431)

SKU:
STJ112431

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZIP8 (180-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC39A8
Gene ID: 64116
Uniprot ID: S39A8_HUMAN
Immunogen Region: 180-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-300 of human ZIP8 (NP_071437.3).
Immunogen Sequence: QLIPEAFGFDPKVDSYVEKA VAVFGGFYLLFFFERMLKML LKTYGQNGHTHFGNDNFGPQ EKTHQPKALPAINGVTCYAN PAVTEANGHIHFDNVSVVSL QDGKKEPSSCTCLKGPKLSE I
Tissue Specificity Ubiquitously expressed. Expressed in thymus, placenta, lung, liver, pancreas, salivary gland and, to a lower extent, in spleen, testis, ovary, small intestine, colon, leukocyte, heart. Highest expression is observed in pancreas. Expressed by macrophages (at protein level). Expressed by microvascular capillary endothelial cells that constitute the blood-brain barrier (at protein level).
Post Translational Modifications N-glycosylated. N-glycosylation is not required for proper iron and zinc transport.
Function Electroneutral divalent metal cation:bicarbonate symporter of the plasma membrane mediating the cellular uptake of zinc and manganese, two divalent metal cations important for development, tissue homeostasis and immunity. Transports an electroneutral complex composed of a divalent metal cation and two bicarbonate anions or alternatively a bicarbonate and a selenite anion. Thereby, it also contributes to the cellular uptake of selenium, an essential trace metal and micronutrient. Also imports cadmium a non-essential metal which is cytotoxic and carcinogenic. May also transport iron and cobalt through membranes. Through zinc import, indirectly regulates the metal-dependent transcription factor MTF1 and the expression of some metalloproteases involved in cartilage catabolism and also probably heart development. Also indirectly regulates the expression of proteins involved in cell morphology and cytoskeleton organization. Indirectly controls innate immune function and inflammatory response by regulating zinc cellular uptake which in turn modulates the expression of genes specific of these processes. Protects, for instance, cells from injury and death at the onset of inflammation. By regulating zinc influx into monocytes also directly modulates their adhesion to endothelial cells and arteries. Reclaims manganese from the bile at the apical membrane of hepatocytes, thereby regulating the activity of the manganese-dependent enzymes through the systemic levels of the nutrient. Also participates in manganese reabsorption in the proximal tubule of the kidney. By mediating the extracellular uptake of manganese by cells of the blood-brain barrier, may also play a role in the transport of the micronutrient to the brain. With manganese cellular uptake also participates in mitochondrial proper function. Finally, also probably functions intracellularly, translocating zinc from lysosome to cytosol to indirectly enhance the expression of specific genes during TCR-mediated T cell activation.
Protein Name Metal Cation Symporter Zip8
Bcg-Induced Integral Membrane Protein In Monocyte Clone 103 Protein
Liv-1 Subfamily Of Zip Zinc Transporter 6
Lzt-Hs6
Solute Carrier Family 39 Member 8
Zrt- And Irt-Like Protein 8
Zip-8
Database Links Reactome: R-HSA-442380
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Lysosome Membrane
Apical Cell Membrane
Basolateral Cell Membrane
Localizes To The Lysosome Of Activated T-Cells
A Large Fraction Of The Protein Is Found Intracellularly In Microvascular Capillary Endothelial Cells That Constitute The Blood-Brain Barrier
Localized And Functional At Both Apical And Basolateral Membranes Of Microvascular Capillary Endothelial Cells That Constitute The Blood-Brain Barrier
Alternative Antibody Names Anti-Metal Cation Symporter Zip8 antibody
Anti-Bcg-Induced Integral Membrane Protein In Monocyte Clone 103 Protein antibody
Anti-Liv-1 Subfamily Of Zip Zinc Transporter 6 antibody
Anti-Lzt-Hs6 antibody
Anti-Solute Carrier Family 39 Member 8 antibody
Anti-Zrt- And Irt-Like Protein 8 antibody
Anti-Zip-8 antibody
Anti-SLC39A8 antibody
Anti-BIGM103 antibody
Anti-ZIP8 antibody
Anti-PP3105 antibody

Information sourced from Uniprot.org

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