• Immunohistochemistry analysis of paraffin-embedded rat liver using ZIP14 Rabbit polyclonal antibody (STJ112448) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SLC39A14 antibody (138-152) (STJ112448)

SKU:
STJ112448

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZIP14 (138-152) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC39A14
Gene ID: 23516
Uniprot ID: S39AE_HUMAN
Immunogen Region: 138-152
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 138-152 of human ZIP14 (NP_001121903.1).
Immunogen Sequence: EENEQTEEGRPSAVE
Tissue Specificity Ubiquitously expressed, with higher expression in liver, pancreas, fetal liver, thyroid gland, left and right ventricle, right atrium and fetal heart. Weakly expressed in spleen, thymus, and peripheral blood leukocytes. Expressed in liver and in brain by large neurons in the globus pallidus, the insular cortex and the dentate nucleus and to a lower extent in the putamen and the caudate nucleus (at protein level). Expressed in osteoblasts and giant osteoclast-like cells, but not in osteocytes found osteoblastoma and giant cell tumors (at protein level). Expressed by microvascular capillary endothelial cells that constitute the blood-brain barrier (at protein level). Expressed by macrophages. Isoform 2: Widely expressed but not detected in brain, heart, skeletal muscle, placenta and fetal skin.
Post Translational Modifications Ubiquitinated. Ubiquitination occurs upon iron depletion. The ubiquitinated form undergoes proteasomal degradation. N-glycosylated. N-glycosylation at Asn-102 is required for iron-regulated extraction of the transporter from membranes and subsequent proteasomal degradation.
Function Electroneutral transporter of the plasma membrane mediating the cellular uptake of the divalent metal cations zinc, manganese and iron that are important for tissue homeostasis, metabolism, development and immunity. Functions as an energy-dependent symporter, transporting through the membranes an electroneutral complex composed of a divalent metal cation and two bicarbonate anions. Beside these endogenous cellular substrates, can also import cadmium a non-essential metal which is cytotoxic and carcinogenic. Controls the cellular uptake by the intestinal epithelium of systemic zinc, which is in turn required to maintain tight junctions and the intestinal permeability. Modifies the activity of zinc-dependent phosphodiesterases, thereby indirectly regulating G protein-coupled receptor signaling pathways important for gluconeogenesis and chondrocyte differentiation. Regulates insulin receptor signaling, glucose uptake, glycogen synthesis and gluconeogenesis in hepatocytes through the zinc-dependent intracellular catabolism of insulin. Through zinc cellular uptake also plays a role in the adaptation of cells to endoplasmic reticulum stress. Major manganese transporter of the basolateral membrane of intestinal epithelial cells, it plays a central role in manganese systemic homeostasis through intestinal manganese uptake. Also involved in manganese extracellular uptake by cells of the blood-brain barrier. May also play a role in manganese and zinc homeostasis participating in their elimination from the blood through the hepatobiliary excretion. Also functions in the extracellular uptake of free iron. May also function intracellularly and mediate the transport from endosomes to cytosol of iron endocytosed by transferrin. Plays a role in innate immunity by regulating the expression of cytokines by activated macrophages.
Protein Name Metal Cation Symporter Zip14
Liv-1 Subfamily Of Zip Zinc Transporter 4
Lzt-Hs4
Solute Carrier Family 39 Member 14
Zrt- And Irt-Like Protein 14
Zip-14
Database Links Reactome: R-HSA-442380
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Basolateral Cell Membrane
Early Endosome Membrane
Late Endosome Membrane
Lysosome Membrane
Localized And Functional At Both Apical And Basolateral Membranes Of Microvascular Capillary Endothelial Cells That Constitute The Blood-Brain Barrier
Localized At The Basolateral Membrane Of Enterocytes
Enriched At The Plasma Membrane Upon Glucose Uptake
Alternative Antibody Names Anti-Metal Cation Symporter Zip14 antibody
Anti-Liv-1 Subfamily Of Zip Zinc Transporter 4 antibody
Anti-Lzt-Hs4 antibody
Anti-Solute Carrier Family 39 Member 14 antibody
Anti-Zrt- And Irt-Like Protein 14 antibody
Anti-Zip-14 antibody
Anti-SLC39A14 antibody
Anti-KIAA0062 antibody
Anti-ZIP14 antibody

Information sourced from Uniprot.org