• Immunofluorescence analysis of paraffin-embedded Mouse skeletal muscle tissue using GLUT4 Rabbit polyclonal antibody (STJ11105583) at a dilution of 1:250 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Hep G2 cells using GLUT4 Rabbit polyclonal antibody (STJ11105583) at a dilution of 1:250 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of GLUT4 in paraffin-embedded Human skeletal muscle tissue using GLUT4 Rabbit polyclonal antibody (STJ11105583) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-SLC2A4 antibody (210-310) (STJ11105583)

SKU:
STJ11105583

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Solute Carrier Family 2-Facilitated Glucose Transporter Member 4 (210-310) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC2A4
Gene ID: 6517
Uniprot ID: GLUT4_HUMAN
Immunogen Region: 210-310
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 210-310 of human GLUT4 (NP_001033.1).
Immunogen Sequence: VLPALLQLVLLPFCPESPRY LYIIQNLEGPARKSLKRLTG WADVSGVLAELKDEKRKLER ERPLSLLQLLGSRTHRQPLI IAVVLQLSQQLSGINAVFYY S
Tissue Specificity Skeletal and cardiac muscles.brown and white fat.
Post Translational Modifications Sumoylated. Palmitoylated. Palmitoylation by ZDHHC7 controls the insulin-dependent translocation of GLUT4 to the plasma membrane.
Function Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell.
Protein Name Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4
Glucose Transporter Type 4 - Insulin-Responsive
Glut-4
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-189200
Reactome: R-HSA-381340
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endomembrane System
Cytoplasm
Perinuclear Region
Localizes Primarily To The Perinuclear Region
Undergoing Continued Recycling To The Plasma Membrane Where It Is Rapidly Reinternalized
The Dileucine Internalization Motif Is Critical For Intracellular Sequestration
Insulin Stimulation Induces Translocation To The Cell Membrane
Alternative Antibody Names Anti-Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4 antibody
Anti-Glucose Transporter Type 4 - Insulin-Responsive antibody
Anti-Glut-4 antibody
Anti-SLC2A4 antibody
Anti-GLUT4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance