• Immunofluorescence analysis of C6 cells using SLC25A1 antibody (STJ112285) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using SLC25A1 antibody (STJ112285) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using SLC25A1 antibody (STJ112285) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using SLC25A1 Rabbit polyclonal antibody (STJ112285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SLC25A1 antibody (1-311) (STJ112285)

SKU:
STJ112285

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC25A1 (1-311) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC25A1
Gene ID: 6576
Uniprot ID: TXTP_HUMAN
Immunogen Region: 1-311
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human SLC25A1 (NP_005975.1).
Immunogen Sequence: MPAPRAPRALAAAAPASGKA KLTHPGKAILAGGLAGGIEI CITFPTEYVKTQLQLDERSH PPRYRGIGDCVRQTVRSHGV LGLYRGLSSLLYGSIPKAAV RFGMFEFLSNHMRDAQGRLD STRGLLCGLGAGVAEAVVVV CPMETIKVKFIHDQTSPNPK YRGFFHGVREIVREQGLKGT YQGLTATVLKQGSNQAIRFF VMTSLRNWYRGDNPNKPMNP LITGVFGAIAGAASVFGNT
Post Translational Modifications Possesses a short cleavable presequence, which, however, is found to be dispensable both for targeting to mitochondria and insertion into the inner membrane. However, the presequence is required to keep SLC25A1 in a soluble state and thus in an import-competent state. Mature SLC25A1 lacking the presequence is prone to aggregation.
Function Mitochondrial electroneutral antiporter that exports citrate from the mitochondria into the cytosol in exchange for malate. Also able to mediate the exchange of citrate for isocitrate, phosphoenolpyruvate, cis-aconitate and to a lesser extend cis-aconitate, maleate and succinate. In the cytoplasm citrate is important in the regulation of glycolysis through a feedback mechanism and in the production of acetyl-CoA which is needed for the synthesis of fatty acids, sterols, prostaglandins, dolichol and coenzyme Q (CoQ). Required for proper neuromuscular junction formation (Probable).
Protein Name Tricarboxylate Transport Protein - Mitochondrial
Citrate Transport Protein
Ctp
Mitochondrial Citrate Carrier
Cic
Solute Carrier Family 25 Member 1
Tricarboxylate Carrier Protein
Database Links Reactome: R-HSA-70263
Reactome: R-HSA-75105
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Tricarboxylate Transport Protein - Mitochondrial antibody
Anti-Citrate Transport Protein antibody
Anti-Ctp antibody
Anti-Mitochondrial Citrate Carrier antibody
Anti-Cic antibody
Anti-Solute Carrier Family 25 Member 1 antibody
Anti-Tricarboxylate Carrier Protein antibody
Anti-SLC25A1 antibody
Anti-SLC20A3 antibody

Information sourced from Uniprot.org

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