| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SLC22A2 (44-140) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLC22A2 |
| Gene ID: |
6582 |
| Uniprot ID: |
S22A2_HUMAN |
| Immunogen Region: |
44-140 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 44-140 of human SLC22A2 (NP_003049.2). |
| Immunogen Sequence: |
GFTPDHRCRSPGVAELSLRC GWSPAEELNYTVPGPGPAGE ASPRQCRRYEVDWNQSTFDC VDPLASLDTNRSRLPLGPCR DGWVYETPGSSIVTEFN |
| Tissue Specificity | Mainly expressed in kidney, in the cortex and medulla. Localized in testis, mostly to peritubular myoid cells and Leydig cells and also detected along the basal membrane of Sertoli cells. Expressed in brain, in neurons of the cerebral cortex and in various subcortical nuclei. In the brain, also detected in the dopaminergic regions of the substantia nigra. Expressed in tracheal and bronchial ciliated epithelium in the respiratory tract. Also detected in secretory phase endometrium, in scattered stromal cells. Expressed in spleen, placenta, small intestine and spinal cord. Weakly expressed in prostate, uterus and lung. Isoform 2: Mainly expressed in kidney, bone marrow and testis. Expressed in colon, skeletal muscle, spinal cord, placenta and liver. |
| Post Translational Modifications | Tyrosine phosphorylated by tyrosine-protein kinase YES1. |
| Function | Electrogenic voltage-dependent transporter that mediates the transport of a variety of organic cations such as endogenous bioactive amines, cationic drugs and xenobiotics. Functions as a Na(+)-independent, bidirectional uniporter. Cation cellular uptake or release is driven by the electrochemical potential, i.e. membrane potential and concentration gradient. However, may also engage electroneutral cation exchange when saturating concentrations of cation substrates are reached. Predominantly expressed at the basolateral membrane of hepatocytes and proximal tubules and involved in the uptake and disposition of cationic compounds by hepatic and renal clearance from the blood flow. Implicated in monoamine neurotransmitters uptake such as histamine, dopamine, adrenaline/epinephrine, noradrenaline/norepinephrine, serotonin and tyramine, thereby supporting a physiological role in the central nervous system by regulating interstitial concentrations of neurotransmitters. Also capable of transporting dopaminergic neuromodulators cyclo(his-pro), salsolinol and N-methyl-salsolinol, thereby involved in the maintenance of dopaminergic cell integrity in the central nervous system. Mediates the bidirectional transport of acetylcholine (ACh) at the apical membrane of ciliated cell in airway epithelium, thereby playing a role in luminal release of ACh from bronchial epithelium. Also transports guanidine and endogenous monoamines such as vitamin B1/thiamine, creatinine and N-1-methylnicotinamide (NMN). Mediates the uptake and efflux of quaternary ammonium compound choline. Mediates the bidirectional transport of polyamine agmatine and the uptake of polyamines putrescine and spermidine. Able to transport non-amine endogenous compounds such as prostaglandin E2 (PGE2) and prostaglandin F2-alpha (PGF2-alpha). Also involved in the uptake of xenobiotic 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP). May contribute to regulate the transport of organic compounds in testis across the blood-testis-barrier (Probable). Isoform 2: In contrast with isoform 1, not able to transport guanidine, creatinine, cimetidine and metformin. |
| Protein Name | Solute Carrier Family 22 Member 2Organic Cation Transporter 2Hoct2 |
| Database Links | Reactome: R-HSA-112311Reactome: R-HSA-181430Reactome: R-HSA-2161517Reactome: R-HSA-442660Reactome: R-HSA-549127 |
| Cellular Localisation | Basolateral Cell MembraneMulti-Pass Membrane ProteinBasal Cell MembraneApical Cell MembraneLocalized To The Basal Membrane Of Sertoli CellsLocalized To The Basolateral Membrane Of Proximal TubuleLocalized To The Luminal/Apical Membrane Of Distal TubuleLocalized To The Luminal/Apical Membrane Of Ciliated Epithelial Cells In Bronchi |
| Alternative Antibody Names | Anti-Solute Carrier Family 22 Member 2 antibodyAnti-Organic Cation Transporter 2 antibodyAnti-Hoct2 antibodyAnti-SLC22A2 antibodyAnti-OCT2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
