• Western blot analysis of extracts of various cell lines, using SIRT3 antibody (STJ29446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded rat heart using SIRT3 antibody (STJ29446) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using SIRT3 antibody (STJ29446) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse heart using SIRT3 antibody (STJ29446) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug SIRT3 antibody (STJ29446). Western blot was performed from the immunoprecipitate using SIRT3 antibody (STJ29446) at a dilition of 1:500.

Anti-SIRT3 antibody (388-399) (STJ29446)

SKU:
STJ29446

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIRT3
Gene ID: 23410
Uniprot ID: SIR3_HUMAN
Immunogen Region: 388-399
Specificity: A synthetic peptide corresponding to a sequence within amino acids 388-399 of human SIRT3 (NP_036371.1).
Immunogen Sequence: QRETGKLDGPDK
Tissue Specificity Widely expressed.
Post Translational Modifications Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
Function NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation. Regulates hepatic lipogenesis. Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by mediating delactylation of proteins, such as CCNE2 and 'Lys-16' of histone H4 (H4K16la).
Protein Name Nad-Dependent Protein Deacetylase Sirtuin-3 - Mitochondrial
Hsirt3
Nad-Dependent Protein Delactylase Sirtuin-3
Regulatory Protein Sir2 Homolog 3
Sir2-Like Protein 3
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-9615017
Reactome: R-HSA-9617629
Reactome: R-HSA-9854311
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Nad-Dependent Protein Deacetylase Sirtuin-3 - Mitochondrial antibody
Anti-Hsirt3 antibody
Anti-Nad-Dependent Protein Delactylase Sirtuin-3 antibody
Anti-Regulatory Protein Sir2 Homolog 3 antibody
Anti-Sir2-Like Protein 3 antibody
Anti-SIRT3 antibody
Anti-SIR2L3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance