| Tissue Specificity | Isoform 1 is expressed in heart, liver and skeletal muscle, weakly expressed in the cortex. Isoform 2 is strongly expressed in the cortex, weakly expressed in heart and liver. Weakly expressed in several malignancies including breast, liver, brain, kidney and prostate cancers compared to normal tissues. Weakly expressed in glioma cell lines compared to normal brain tissues (at protein level). Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. |
| Post Translational Modifications | Phosphorylated at phosphoserine and phosphothreonine. Phosphorylated at Ser-368 by a mitotic kinase CDK1/cyclin B at the G2/M transition.phosphorylation regulates the delay in cell-cycle progression. Phosphorylated at Ser-368 by a mitotic kinase G1/S-specific cyclin E/Cdk2 complex.phosphorylation inactivates SIRT2-mediated alpha-tubulin deacetylation and thereby negatively regulates cell adhesion, cell migration and neurite outgrowth during neuronal differentiation. Phosphorylated by cyclin A/Cdk2 and p35-Cdk5 complexes and to a lesser extent by the cyclin D3/Cdk4 and cyclin B/Cdk1, in vitro. Dephosphorylated at Ser-368 by CDC14A and CDC14B around early anaphase. Acetylated by EP300.acetylation leads both to the decreased of SIRT2-mediated alpha-tubulin deacetylase activity and SIRT2-mediated down-regulation of TP53 transcriptional activity. Ubiquitinated. |
| Function | NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and alpha-tubulin as well as many other proteins such as key transcription factors. Participates in the modulation of multiple and diverse biological processes such as cell cycle control, genomic integrity, microtubule dynamics, cell differentiation, metabolic networks, and autophagy. Plays a major role in the control of cell cycle progression and genomic stability. Functions in the antephase checkpoint preventing precocious mitotic entry in response to microtubule stress agents, and hence allowing proper inheritance of chromosomes. Positively regulates the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex activity by deacetylating CDC20 and FZR1, then allowing progression through mitosis. Associates both with chromatin at transcriptional start sites (TSSs) and enhancers of active genes. Plays a role in cell cycle and chromatin compaction through epigenetic modulation of the regulation of histone H4 'Lys-20' methylation (H4K20me1) during early mitosis. Specifically deacetylates histone H4 at 'Lys-16' (H4K16ac) between the G2/M transition and metaphase enabling H4K20me1 deposition by KMT5A leading to ulterior levels of H4K20me2 and H4K20me3 deposition throughout cell cycle, and mitotic S-phase progression. Deacetylates KMT5A modulating KMT5A chromatin localization during the mitotic stress response. Deacetylates also histone H3 at 'Lys-57' (H3K56ac) during the mitotic G2/M transition. Upon bacterium Listeria monocytogenes infection, deacetylates 'Lys-18' of histone H3 in a receptor tyrosine kinase MET- and PI3K/Akt-dependent manner, thereby inhibiting transcriptional activity and promoting late stages of listeria infection. During oocyte meiosis progression, may deacetylate histone H4 at 'Lys-16' (H4K16ac) and alpha-tubulin, regulating spindle assembly and chromosome alignment by influencing microtubule dynamics and kinetochore function. Deacetylates histone H4 at 'Lys-16' (H4K16ac) at the VEGFA promoter and thereby contributes to regulate expression of VEGFA, a key regulator of angiogenesis. Deacetylates alpha-tubulin at 'Lys-40' and hence controls neuronal motility, oligodendroglial cell arbor projection processes and proliferation of non-neuronal cells. Phosphorylation at Ser-368 by a G1/S-specific cyclin E-CDK2 complex inactivates SIRT2-mediated alpha-tubulin deacetylation, negatively regulating cell adhesion, cell migration and neurite outgrowth during neuronal differentiation. Deacetylates PARD3 and participates in the regulation of Schwann cell peripheral myelination formation during early postnatal development and during postinjury remyelination. Involved in several cellular metabolic pathways. Plays a role in the regulation of blood glucose homeostasis by deacetylating and stabilizing phosphoenolpyruvate carboxykinase PCK1 activity in response to low nutrient availability. Acts as a key regulator in the pentose phosphate pathway (PPP) by deacetylating and activating the glucose-6-phosphate G6PD enzyme, and therefore, stimulates the production of cytosolic NADPH to counteract oxidative damage. Maintains energy homeostasis in response to nutrient deprivation as well as energy expenditure by inhibiting adipogenesis and promoting lipolysis. Attenuates adipocyte differentiation by deacetylating and promoting FOXO1 interaction to PPARG and subsequent repression of PPARG-dependent transcriptional activity. Plays a role in the regulation of lysosome-mediated degradation of protein aggregates by autophagy in neuronal cells. Deacetylates FOXO1 in response to oxidative stress or serum deprivation, thereby negatively regulating FOXO1-mediated autophagy. Deacetylates a broad range of transcription factors and co-regulators regulating target gene expression. Deacetylates transcriptional factor FOXO3 stimulating the ubiquitin ligase SCF(SKP2)-mediated FOXO3 ubiquitination and degradation. Deacetylates HIF1A and therefore promotes HIF1A degradation and inhibition of HIF1A transcriptional activity in tumor cells in response to hypoxia. Deacetylates RELA in the cytoplasm inhibiting NF-kappaB-dependent transcription activation upon TNF-alpha stimulation. Inhibits transcriptional activation by deacetylating p53/TP53 and EP300. Deacetylates also EIF5A. Functions as a negative regulator on oxidative stress-tolerance in response to anoxia-reoxygenation conditions. Plays a role as tumor suppressor. In addition to protein deacetylase activity, also has activity toward long-chain fatty acyl groups and mediates protein-lysine demyristoylation and depalmitoylation of target proteins, such as ARF6 and KRAS, thereby regulating their association with membranes. Isoform 1: Deacetylates EP300, alpha-tubulin and histone H3 and H4. Isoform 2: Deacetylates EP300, alpha-tubulin and histone H3 and H4. Isoform 5: Lacks deacetylation activity, at least toward known SIRT2 targets. |
| Protein Name | Nad-Dependent Protein Deacetylase Sirtuin-2Nad-Dependent Protein Defatty-Acylase Sirtuin-2Regulatory Protein Sir2 Homolog 2Sir2-Like Protein 2 |
| Database Links | Reactome: R-HSA-2995383 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleSpindleMidbodyChromosomePerikaryonCell ProjectionGrowth ConeMyelin MembraneLocalizes In The Cytoplasm During Most Of The Cell Cycle Except In The G2/M Transition And During MitosisWhere It Is Localized In Association With Chromatin And Induces Deacetylation Of Histone At 'Lys-16' (H4k16ac)Colocalizes With Kmt5a At Mitotic FociColocalizes With Cdk1 At Centrosome During Prophase And Splindle Fibers During MetaphaseColocalizes With Aurora Kinase Aurka At Centrosome During Early Prophase And In The Centrioles And Growing Mitotic Spindle Throughout MetaphaseColocalizes With Aurora Kinase Aurkb During Cytokinesis With The MidbodyColocalizes With MicrotubulesDetected In Perinuclear Foci That May Be Aggresomes Containing MisfoldedUbiquitinated ProteinsShuttles Between The Cytoplasm And The Nucleus Through The Crm1 Export PathwayColocalizes With Ep300 In The NucleusTranslocates To The Nucleus And Chromatin Upon Bacterium Listeria Monocytogenes Infection In Interphase CellsDeacetylates Foxo3 In The CytoplasmColocalizes With Plp1 In Internodal RegionsAt Paranodal Axoglial Junction And Schmidt-Lanterman Incisures Of Myelin SheathColocalizes With Cdk5r1 In The PerikaryonNeurites And Growth Cone Of Hippocampal NeuronsColocalizes With Alpha-Tubulin In Neuronal Growth ConeLocalizes In The Cytoplasm And Nucleus Of Germinal Vesicle (Gv) Stage OocytesColocalizes With Alpha-Tubulin On The Meiotic Spindle As The Oocytes Enter Into MetaphaseAnd Also During Meiotic Anaphase And TelophaseEspecially With The MidbodyColocalizes With Pard3 In Internodal Region Of AxonsColocalizes With Acetylated Alpha-Tubulin In Cell Projection Processes During Primary Oligodendrocyte Precursor (Olp) DifferentiationIsoform 1: CytoplasmPredominantly Localized In The CytoplasmicIsoform 2: CytoplasmIsoform 5: CytoplasmPredominantly Localized In The Nucleus |
| Alternative Antibody Names | Anti-Nad-Dependent Protein Deacetylase Sirtuin-2 antibodyAnti-Nad-Dependent Protein Defatty-Acylase Sirtuin-2 antibodyAnti-Regulatory Protein Sir2 Homolog 2 antibodyAnti-Sir2-Like Protein 2 antibodyAnti-SIRT2 antibodyAnti-SIR2L antibodyAnti-SIR2L2 antibody |
