Anti-SIRP-Alpha 1/Beta 1 antibody (281-330 aa) (STJ97319)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human SIRPA/SIRPB1 at the amino acid range 281-330
STJ97319
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General Information

Short DescriptionRabbit polyclonal anti-Tyrosine-protein phosphatase non-receptor type substrate 1 and Signal-regulatory protein beta-1 isoform 3 (281-330 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommend
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman/Rat/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
IHC-P 1:100-1:300
ELISA 1:10000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSIRPA
SIRPB1
Gene ID140885
10326
Uniprot IDSHPS1_HUMAN
SIRBL_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human SIRPA/SIRPB1 at the amino acid range 281-330
Immunogen Region281-330 aa
SpecificitySIRP-Alpha 1/Beta 1 Polyclonal Antibody detects endogenous levels of SIRP-Alpha 1/Beta 1 protein.

Additional Info

Post Translational Modifications N-glycosylated. Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
Function Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1
Shp Substrate 1
Shps-1
Brain Ig-Like Molecule With Tyrosine-Based Activation Motifs
Bit
Cd172 Antigen-Like Family Member A
Inhibitory Receptor Shps-1
Macrophage Fusion Receptor
Myd-1 Antigen
Signal-Regulatory Protein Alpha-1
Sirp-Alpha-1
Signal-Regulatory Protein Alpha-2
Sirp-Alpha-2
Signal-Regulatory Protein Alpha-3
Sirp-Alpha-3
P84
Cd Antigen Cd172a
Database Links Reactome: R-HSA-202733
Reactome: R-HSA-391160
Reactome: R-HSA-6798695
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1 antibody
Anti-Shp Substrate 1 antibody
Anti-Shps-1 antibody
Anti-Brain Ig-Like Molecule With Tyrosine-Based Activation Motifs antibody
Anti-Bit antibody
Anti-Cd172 Antigen-Like Family Member A antibody
Anti-Inhibitory Receptor Shps-1 antibody
Anti-Macrophage Fusion Receptor antibody
Anti-Myd-1 Antigen antibody
Anti-Signal-Regulatory Protein Alpha-1 antibody
Anti-Sirp-Alpha-1 antibody
Anti-Signal-Regulatory Protein Alpha-2 antibody
Anti-Sirp-Alpha-2 antibody
Anti-Signal-Regulatory Protein Alpha-3 antibody
Anti-Sirp-Alpha-3 antibody
Anti-P84 antibody
Anti-Cd Antigen Cd172a antibody
Anti-SIRPA antibody
Anti-BIT antibody
Anti-MFR antibody
Anti-MYD1 antibody
Anti-PTPNS1 antibody
Anti-SHPS1 antibody
Anti-SIRP antibody

Information sourced from Uniprot.org

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