• Western blot analysis of various lysates, using SIM2 Rabbit polyclonal antibody (STJ117292) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SIM2 antibody (342-525) (STJ117292)

SKU:
STJ117292

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIM2
Gene ID: 6493
Uniprot ID: SIM2_HUMAN
Immunogen Region: 342-525
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 342-525 of human SIM2 (NP_005060.1?).
Immunogen Sequence: LSLEQVSTAKSQDSWRTALS TSQETRKLVKPKNTKMKTKL RTNPYPPQQYSSFQMDKLEC GQLGNWRASPPASAAAPPEL QPHSESSDLLYTPSYSLPFS YHYGHFPLDSHVFSSKKPML PAKFGQPQGSPCEVARFFLS TLPASGECQWHYANPLVPSS SSPAKNPPEPPANTARHSLV PSYE
Function Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.
Protein Name Single-Minded Homolog 2
Class E Basic Helix-Loop-Helix Protein 15
Bhlhe15
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Single-Minded Homolog 2 antibody
Anti-Class E Basic Helix-Loop-Helix Protein 15 antibody
Anti-Bhlhe15 antibody
Anti-SIM2 antibody
Anti-BHLHE15 antibody

Information sourced from Uniprot.org

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