| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SIGMAR1 (114-223) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:100-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SIGMAR1 |
| Gene ID: |
10280 |
| Uniprot ID: |
SGMR1_HUMAN |
| Immunogen Region: |
114-223 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 114-223 of human SIGMAR1 (NP_005857.1). |
| Immunogen Sequence: |
RGHSGRYWAEISDTIISGTF HQWREGTTKSEVFYPGETVV HGPGEATAVEWGPNTWMVEY GRGVIPSTLAFALADTVFST QDFLTLFYTLRSYARGLRLE LTTYLFGQDP |
| Tissue Specificity | Widely expressed with higher expression in liver, colon, prostate, placenta, small intestine, heart and pancreas. Expressed in the retina by retinal pigment epithelial cells. Expressed in alpha-motor neurons. |
| Function | Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration. Necessary for proper mitochondrial axonal transport in motor neurons, in particular the retrograde movement of mitochondria. Plays a role in protecting cells against oxidative stress-induced cell death via its interaction with RNF112. |
| Protein Name | Sigma Non-Opioid Intracellular Receptor 1Aging-Associated Gene 8 ProteinSr31747-Binding ProteinSr-BpSigma 1-Type Opioid ReceptorSig-1rSigma1-ReceptorSigma1rHsigmar1 |
| Database Links | Reactome: R-HSA-9679191 |
| Cellular Localisation | Nucleus Inner MembraneNucleus Outer MembraneNucleus EnvelopeCytoplasmic VesicleEndoplasmic Reticulum MembraneMembraneSingle-Pass Membrane ProteinLipid DropletCell JunctionCell MembraneCell ProjectionGrowth ConePostsynaptic Density MembraneDuring InterphaseDetected At The Inner And Outer Nuclear Membrane And The Endoplasmic ReticulumDetected On Cytoplasmic Vesicles During MitosisTargeted To Lipid DropletsCholesterol And Galactosylceramide-Enriched Domains Of The Endoplasmic ReticulumAccumulation At The Endoplasmic Reticulum Is Prominent In Alpha-Motor Neurons Of Patients With Amyotrophic Lateral SclerosisEnriched At Cell-Cell Communication RegionsGrowth Cone And Postsynaptic StructuresLocalization Is Modulated By Ligand-BindingIn Motor Neurons It Is Enriched At Cholinergic Postsynaptic Densities |
| Alternative Antibody Names | Anti-Sigma Non-Opioid Intracellular Receptor 1 antibodyAnti-Aging-Associated Gene 8 Protein antibodyAnti-Sr31747-Binding Protein antibodyAnti-Sr-Bp antibodyAnti-Sigma 1-Type Opioid Receptor antibodyAnti-Sig-1r antibodyAnti-Sigma1-Receptor antibodyAnti-Sigma1r antibodyAnti-Hsigmar1 antibodyAnti-SIGMAR1 antibodyAnti-OPRS1 antibodyAnti-SRBP antibodyAnti-AAG8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89668/358164/STJ11100730_1__42931.1713122467.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
