• Western blot analysis of lysates from HeLa cells, using SIGMAR1 Rabbit polyclonal antibody (STJ117037) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SIGMAR1 antibody (31-80) (STJ117037)

SKU:
STJ117037

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SIGMAR1 (31-80) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIGMAR1
Gene ID: 10280
Uniprot ID: SGMR1_HUMAN
Immunogen Region: 31-80
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 31-80 of human SIGMAR1 (NP_005857.1).
Immunogen Sequence: GTQSFVFQREEIAQLARQYA GLDHELAFSRLIVELRRLHP GHVLPDEELQ
Tissue Specificity Widely expressed with higher expression in liver, colon, prostate, placenta, small intestine, heart and pancreas. Expressed in the retina by retinal pigment epithelial cells. Expressed in alpha-motor neurons.
Function Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration. Necessary for proper mitochondrial axonal transport in motor neurons, in particular the retrograde movement of mitochondria. Plays a role in protecting cells against oxidative stress-induced cell death via its interaction with RNF112.
Protein Name Sigma Non-Opioid Intracellular Receptor 1
Aging-Associated Gene 8 Protein
Sr31747-Binding Protein
Sr-Bp
Sigma 1-Type Opioid Receptor
Sig-1r
Sigma1-Receptor
Sigma1r
Hsigmar1
Database Links Reactome: R-HSA-9679191
Cellular Localisation Nucleus Inner Membrane
Nucleus Outer Membrane
Nucleus Envelope
Cytoplasmic Vesicle
Endoplasmic Reticulum Membrane
Membrane
Single-Pass Membrane Protein
Lipid Droplet
Cell Junction
Cell Membrane
Cell Projection
Growth Cone
Postsynaptic Density Membrane
During Interphase
Detected At The Inner And Outer Nuclear Membrane And The Endoplasmic Reticulum
Detected On Cytoplasmic Vesicles During Mitosis
Targeted To Lipid Droplets
Cholesterol And Galactosylceramide-Enriched Domains Of The Endoplasmic Reticulum
Accumulation At The Endoplasmic Reticulum Is Prominent In Alpha-Motor Neurons Of Patients With Amyotrophic Lateral Sclerosis
Enriched At Cell-Cell Communication Regions
Growth Cone And Postsynaptic Structures
Localization Is Modulated By Ligand-Binding
In Motor Neurons It Is Enriched At Cholinergic Postsynaptic Densities
Alternative Antibody Names Anti-Sigma Non-Opioid Intracellular Receptor 1 antibody
Anti-Aging-Associated Gene 8 Protein antibody
Anti-Sr31747-Binding Protein antibody
Anti-Sr-Bp antibody
Anti-Sigma 1-Type Opioid Receptor antibody
Anti-Sig-1r antibody
Anti-Sigma1-Receptor antibody
Anti-Sigma1r antibody
Anti-Hsigmar1 antibody
Anti-SIGMAR1 antibody
Anti-OPRS1 antibody
Anti-SRBP antibody
Anti-AAG8 antibody

Information sourced from Uniprot.org

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