• Western blot analysis of extracts of various cell lines, using SIGLEC12 antibody (STJ110817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using SIGLEC12 antibody (STJ110817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SIGLEC12 antibody (20-210) (STJ110817)

SKU:
STJ110817

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SIGLEC12 (20-210) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIGLEC12
Gene ID: 89858
Uniprot ID: SIG12_HUMAN
Immunogen Region: 20-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-210 of human SIGLEC12 (NP_443729.1).
Immunogen Sequence: EQKDYLLTMQKSVTVQEGLC VSVLCSFSYPQNGWTASDPV HGYWFRAGDHVSRNIPVATN NPARAVQEETRDRFHLLGDP QNKDCTLSIRDTRESDAGTY VFCVERGNMKWNYKYDQLSV NVTASQDLLSRYRLEVPESV TVQEGLCVSVPCSVLYPHYN WTASSPVYGSWFKEGADIPW DIPVATNTPSG
Tissue Specificity Isoform Short is highly expressed in spleen, small intestine and adrenal gland.it is lower expressed in thyroid, placenta, brain, stomach, bone marrow, spinal cord and breast. Isoform Long is highly expressed in spleen, small intestine and bone marrow.it is lower expressed in thyroid, placenta, thymus, trachea, stomach, lung, adrenal gland, fetal brain and testis.
Function Putative adhesion molecule that mediates sialic-acid dependent binding to cells. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Protein Name Sialic Acid-Binding Ig-Like Lectin 12
Siglec-12
Sialic Acid-Binding Ig-Like Lectin-Like 1
Siglec-L1
Database Links Reactome: R-HSA-198933
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Sialic Acid-Binding Ig-Like Lectin 12 antibody
Anti-Siglec-12 antibody
Anti-Sialic Acid-Binding Ig-Like Lectin-Like 1 antibody
Anti-Siglec-L1 antibody
Anti-SIGLEC12 antibody
Anti-SIGLECL1 antibody
Anti-SLG antibody
Anti-UNQ9215 antibody
Anti-PRO34042 antibody

Information sourced from Uniprot.org

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