• Western blot analysis of extracts of various cell lines, using SHPK antibody (STJ116331) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SHPK antibody (1-320) (STJ116331)

SKU:
STJ116331

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SHPK (1-320) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SHPK
Gene ID: 23729
Uniprot ID: SHPK_HUMAN
Immunogen Region: 1-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human SHPK (NP_037408.2).
Immunogen Sequence: MAARPITLGIDLGTTSVKAA LLRAAPDDPSGFAVLASCAR AARAEAAVESAVAGPQGREQ DVSRILQALHECLAALPRPQ LRSVVGIGVSGQMHGVVFWK TGQGCEWTEGGITPVFEPRA VSHLVTWQDGRCSSEFLASL PQPKSHLSVATGFGCATIFW LLKYRPEFLKSYDAAGTIHD YVVAMLCGLPRPLMSDQNAA SWGYFNTQSQSWNVETLRSS GFPVHLLPDIAEPGSVAGR
Tissue Specificity Strongly expressed in liver, kidney and pancreas. Expressed at lower levels in placenta and heart. Very weakly expressed in lung and brain.
Function Acts as a modulator of macrophage activation through control of glucose metabolism.
Protein Name Sedoheptulokinase
Shk
Carbohydrate Kinase-Like Protein
Database Links Reactome: R-HSA-71336
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Sedoheptulokinase antibody
Anti-Shk antibody
Anti-Carbohydrate Kinase-Like Protein antibody
Anti-SHPK antibody
Anti-CARKL antibody

Information sourced from Uniprot.org

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