• Western blot analysis of extracts of various cell lines, using SHOX antibody (STJ11103031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using SHOX antibody (STJ11103031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SHOX antibody (20-110) (STJ11103031)

SKU:
STJ11103031

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SHOX
Gene ID: 6473
Uniprot ID: SHOX_HUMAN
Immunogen Region: 20-110
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human SHOX (NP_000442.1).
Immunogen Sequence: NGGGGGGGGKKDSITYREVL ESGLARSRELGTSDSSLQDI TEGGGHCPVHLFKDHVDNDK EKLKEFGTARVAEGIYECKE KREDVKSEDED
Tissue Specificity SHOXA is expressed in skeletal muscle, placenta, pancreas, heart and bone marrow fibroblast and SHOXB is highly expressed in bone marrow fibroblast followed by kidney and skeletal muscle. SHOXB is not expressed in brain, kidney, liver and lung. Highly expressed in osteogenic cells.
Function Controls fundamental aspects of growth and development.
Protein Name Short Stature Homeobox Protein
Pseudoautosomal Homeobox-Containing Osteogenic Protein
Short Stature Homeobox-Containing Protein
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Short Stature Homeobox Protein antibody
Anti-Pseudoautosomal Homeobox-Containing Osteogenic Protein antibody
Anti-Short Stature Homeobox-Containing Protein antibody
Anti-SHOX antibody
Anti-PHOG antibody

Information sourced from Uniprot.org

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